Decolorization potential of bacteria isolated from Sungai Lembing hot springs and Ganoderma lucidum on methyl red dye

• Main Authors: Hafidz, Ismail Fitri Mohd, Ramli, Muhamad Syaffuan, Abdullah, Nur Raihan, Wan-Mohtar, Wan Abd Al Qadr Imad, Azizan, Nur Hafizah, Sharif, Faez
• Format: Conference Proceeding
• Language: English
• Subjects: Bacteria; Biodegradation; Decoloring; Dyes; Functional groups; Hot springs; Industrial effluents; Metabolites; Mushrooms; Spectrophotometry; Spectrum analysis; Substrates; White rot fungi
• ISSN: 0094-243X; 1551-7616
• DOI: 10.1063/5.0078615

Two bacteria strains isolated from Sungai Lembing hot springs, Pahang, Malaysia (Achromobacter marplatensis and Aquamicrobium defluvii) and a white-rot fungus (Ganoderma lucidum) were tested for their decolorization potential. The microbes were developed in minimal salt medium (MSM) supplemented with co-substrate (glucose) and 100 mg/L methyl red dye under 30°C and 37°C with agitation for the decolorization treatment. The dye decolorization rate was spectrophotometrically determined within 3 stipulated treatment days for both temperatures and the colour changes between those samples were observed. G. lucidum effectively decolorized methyl red dye with 64.14% and 33.01% at 30°C and 37°C, respectively. The other strains, Achromobacter marplatensis and Aquamicrobium defluvii were effective at 37C with the decolorizing efficiency of 29.18% and 6.04%, respectively. Further comparative analysis of all metabolites and control through the FTIR spectral analysis depicted a slight change of functional groups of the methyl red dye, indicating the biodegradation activity of the samples. Thus, this study showed G. lucidum had higher potential for methyl red dye decolorization in both different temperatures compared to the hot springs’ bacteria, hence indicates its potential to be used in the industrial effluents’ treatment containing azo dye.