Evaluation of Averrhoa bilimbi fruit filtrate as a carrier of lactic acid bacteria for poultry

• Main Authors: Sugiharto, S., Pratama, Anugrah R., Yudiarti, Turrini, Wahyuni, Hanny I., Widiastuti, Endang, Sartono, Tri A.
• Format: Conference Proceeding
• Language: English
• Subjects: Antioxidants; Ascorbic acid; Bacteria; Lactic acid; Vitamin C
• ISSN: 0094-243X; 1551-7616
• DOI: 10.1063/5.0124911

The study evaluated the potential of Averrhoa bilimbi fruit filtrate (ABFF) as a carrier of lactic acid bacteria (LAB). LAB isolates were inoculated into ABFF, incubated, and pH and LAB were determined. The LAB-inoculated ABFF were stored at ±25°C or ±4°C. On days 4, 15 and 30, the culture was sampled and determined for LAB, pH, lactic acid, vitamin C and antioxidant capacity. As a control, LAB isolates were inoculated in deMan Rogosa Sharpe broth and stored at the same temperatures and periods. The LAB linearly increased, while pH decreased with incubation period. The type of LAB medium exerted no effect on LAB counts. The pH was lower in LAB-inoculated ABFF than that in LABinoculated MRS broth. Compared to MRS broth, LAB-inoculated ABFF showed higher vitamin C, and that the concentration decreased with the incubation temperature and storage. The 2,2′-azino-bis(3-ethyl-benzthiazolin-6-sulfonicacid) inhibition percentage was higher in LAB-inoculated MRS broth than that of ABFF. Incubation temperature reduced the antioxidant capacity of the LAB-inoculated culture. Overall, ABFF was able to support the growth of LAB, and could therefore be alternative as LAB culture medium. The presence of vitamin C and antioxidants in filtrate supported the potential of ABFF as a carrier of LAB.