The role of neuroprotection in the zebrafish (Danio rerio) animal model

• Main Authors: Rinendyaputri, Ratih, Lienggonegoro, Lisa Andriani, Idrus, Hasta Handayani, Noverina, Rachmawati, Faried, Ahmad
• Format: Conference Proceeding
• Language: English
• Subjects: Biocompatibility; Cell death; Pathogenesis; Toxic diseases; Vertebrates; Zebrafish
• ISSN: 0094-243X; 1551-7616
• DOI: 10.1063/5.0176280

Zebrafish (Danio rerio) can be used as an alternative animal model in neuroscience research. Zebrafish are vertebrate animals that have 90% more genetic similarities and various pathways with humans. The development of embryos to adults is experienced outside the body, making it easier to observe when giving treatment during research. Observations of anatomy, molecular, genetic, behavioral and cognitive levels can be assessed and observed during the study. Making stroke models, Alzeimer’s and Parkinson’s can be used to get new drugs, new drug toxicity, pathogenesis of a disease and drug mechanisms against a disease. Therapy using neurotrophic factors such as brain derived neurotrophic factor/BDNF has been reported as anti-inflammatory and prevents neuronal cell death or neuroprotection. Therapeutic effects can be done by analyzing transcriptomically and observing changes in behavior. Disturbances in the fish’s body can be seen in their behavior and cognitive spatial. To determine the conditions can be done by using a camera with automatic software that records of all fish activities. The use of zebrafish as a model animal can reduce the 3Rs principle (reduction) in the use of larger animal models. In addition, the translational research gap from in vitro drug discover trials to clinical trials can be narrowed. Preclinical trials in vivo on the right animal models can provide better and more accurate information.