Germplasm screening of diploid Musa spp. collection for fusarium wilt disease resistance using multiplex-SCAR markers

• Main Authors: Zaelani, Ahmad, Herlina, Poerba, Yuyu Suryasari
• Format: Conference Proceeding
• Language: English
• Subjects: Bananas; Domestication; Fusarium oxysporum; Multiplexing; Research facilities
• ISSN: 0094-243X; 1551-7616
• DOI: 10.1063/5.0185779

Banana is one of the major fruit commodities in the world. However, the production of bananas in the world is currently being disrupted by Panama Disease or Fusarium Wilt Disease (FWD), a major disease and the most dangerous disease in bananas. This disease is caused by Fusarium oxysporum f. sp. cubense (Foc), which infects the root system and eventually causes severe chlorosis and wilting. In Indonesia, FWD was detected first in the 1990s in Java, then spread widely to other banana plantations in other Indonesian regions. Currently, it has been studied and reported that there were some wild cultivars/local bananas which have resistance traits to FWD, but the number of FWD-resistant banana accessions is still limited. As the centre of origin and diversity of bananas in the world, Indonesia has a big potential to have more resistant banana accessions to the FWD. In this study, we screened eighty-nine diploid (2x) bananas which consisted of twenty-five wild banana-seeded and sixty-four domesticated bananas from several areas of Indonesia. The plant materials were collected from the exploration program of the Research Center for Biology-LIPI (currently National Research and Innovation Agency/BRIN) from 2009-2019. Two SCAR primers (ScaS0901 and ScaU1001) were used and were multiplexed with an 18S rRNA gene primer. Twenty-nine (45.3%) of sixty-four domesticated banana accessions revealed have resistance trait to FWD based on the presence of the two SCAR marker bands. In addition, among twenty-five wild banana accessions, three wild banana-seeded accessions were indicated as resistant to this disease. This study is the first report for an early-detection study of FWD in Indonesian bananas by using a multiplex SCAR marker system. The genetic information of this study will be beneficial for the banana breeding program, especially in developing a new banana cultivar which has resistance traits to Fusarium Wilt Disease.