Comparison of decellularization protocols for preparing an adipose tissue derived acellular scaffold for soft tissue engineering

• Main Authors: Pokrywczyńska, Marta, Skrzydlewski, Paweł, Fekner, Zuzanna, Buhl, Monika, Drewa, Tomasz, Jundziłł, Arkadiusz
• Format: Conference Proceeding
• Language: English
• Subjects: Adipose tissue; Biocompatibility; Body fat; Cytotoxicity; Effectiveness; In vivo methods and tests; Lipids; Mechanical properties; Mechanical tests; Modulus of elasticity; Regeneration (physiology); Scaffolds; Soft tissues; Staining; Strain; Tissue engineering; Toxicity testing
• ISSN: 0094-243X; 1551-7616
• DOI: 10.1063/5.0216727

The aim of this study was to elaborate a decellularization protocol of adipose tissue for soft tissue engineering. For this purpose, three selected decellularization protocols were compared regarding the effectiveness of cell and lipid removal, cytotoxicity and mechanical properties of obtained adipose tissue derived extracellular matrix (AT-ECM). Porcine adipose tissue was decellularized using three protocols: 1: Brown’s, 2: Wang’s and 3. Roehm’s. Decellularization effectiveness was assessed by H&E staining. Additionally, the lipids content was evaluated by Oil Red O staining. AT-ECM cytotoxicity was analysed using an MTT assay. The Young’s modulus and stress values at 20% strain were calculated. The study indicated that the 2nd protocol removed cells the most effectively. AT-ECM obtained using the 1st and 2nd protocols were characterized by low cytotoxicity in contrast to the 3rd protocol. None of the compared protocols removed the lipids completely. The mechanical test showed that the most resilient were the scaffolds obtained using the 1st and 2ndprotocols. In conclusion, the best among the three compared adipose tissue decellularization protocols was the 2nd protocol. The AT-ECM scaffolds obtained with this method can be used in the future studies on soft tissue regeneration in vivo.