HLA Typing Using DNA Probes

We discuss the genetic organization of the human major histocompatibility complex (the HLA region), and review recent advances in HLA typing achieved by analysis at the DNA level. The use of HLA Class II DNA probes, labelled either with 32 P or non–radioactively, has revealed polymorphisms not detec...

Full description

Saved in:
Bibliographic Details
Published in:Bio/Technology 1986-11, Vol.4 (11), p.975-981
Main Authors: Erlich, Henry A, Sheldon, Edward L, Horn, Glenn
Format: Article
Language:English
Subjects:
Agriculture
Bioinformatics
Biological and medical sciences
Biomedical and Life Sciences
Biomedical Engineering/Biotechnology
Biomedicine
Biotechnology
Fundamental and applied biological sciences. Psychology
Gene therapy
Health. Pharmaceutical industry
Industrial applications and implications. Economical aspects
Life Sciences
review-article
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We discuss the genetic organization of the human major histocompatibility complex (the HLA region), and review recent advances in HLA typing achieved by analysis at the DNA level. The use of HLA Class II DNA probes, labelled either with 32 P or non–radioactively, has revealed polymorphisms not detectable with conventional serologic typing reagents. These DNA polymorphisms, identified by restriction fragment length analyses, or by allele–specific oligonucleotide probes, have proved informative in transplantation tissue–typing, paternity determinations, and for disease susceptibility studies.
ISSN:0733-222X
2331-3684
1546-1696
DOI:10.1038/nbt1186-975