Phytophthora capsici on vegetable hosts in South Africa: distribution, host range and genetic diversity

The oomycete Phytophthora capsici is an economically important plant pathogen worldwide. Not much is known about this pathogen in South Africa. Therefore, the first aim of the study was to obtain a better understanding of the host range and distribution of the pathogen. Second, South African P. caps...

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Bibliographic Details
Published in:Australasian plant pathology 2010-01, Vol.39 (5), p.431-439
Main Authors: Meitz, Julia C, Linde, Celeste C, Thompson, Alistair, Langenhoven, Shaun, McLeod, Adéle
Format: Article
Language:English
Subjects:
Agriculture
Asexual reproduction
Biomedical and Life Sciences
blight
Capsicum
Crops
Cucumis melo
Cucurbita
Ecology
Entomology
Genetic diversity
Genetic markers
Genomes
Genotypes
Genotyping
Host range
Life Sciences
Lycopersicon esculentum
Mating types
Microsatellites
mortality
Nucleotide sequence
Oomycetes
Pathogens
Phytophthora capsici
Plant Pathology
Plant Sciences
Sex
Simple sequence repeats
survival
temperate climate
temperate zones
Vegetables
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Summary:The oomycete Phytophthora capsici is an economically important plant pathogen worldwide. Not much is known about this pathogen in South Africa. Therefore, the first aim of the study was to obtain a better understanding of the host range and distribution of the pathogen. Second, South African P. capsici isolates were characterised through mating-type tests and genotyping with eight microsatellite markers (simple sequence repeat markers (SSR)) that were developed from the draft genome sequence. Analyses of diseased samples from 2000 to 2008 showed that P. capsici was present in at least five provinces on cucurbitaceous ( Cucumis melo and Cucurbita spp.) and solanaceous ( Capsicum spp. and Lycopersicon esculentum ) crops. Mating-type analyses of 78 isolates collected from these hosts showed that both mating types were present in all the sampled production regions. The mating-type frequency in the total South African population did not deviate significantly from a 1 : 1 ratio. Microsatellite genotyping of the isolates revealed that genotypic diversity was high in all regional populations with 59 of 78 isolates representing unique SSR multilocus genotypes (MLG). The remaining 19 isolates consisted of eight MLG that each included two to three isolates with identical SSR alleles. These identical isolates were considered to be clones that were derived from asexual reproduction due to significant P SEX
ISSN:0815-3191
0156-0972
1448-6032
DOI:10.1071/AP09075