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Phytophthora capsici on vegetable hosts in South Africa: distribution, host range and genetic diversity
The oomycete Phytophthora capsici is an economically important plant pathogen worldwide. Not much is known about this pathogen in South Africa. Therefore, the first aim of the study was to obtain a better understanding of the host range and distribution of the pathogen. Second, South African P. caps...
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| Published in: | Australasian plant pathology 2010-01, Vol.39 (5), p.431-439 |
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| Main Authors: | , , , , |
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| cites | cdi_FETCH-LOGICAL-c396t-140651efe1535ffaa98fde174a6bd2c6ab69b07665aac99a3323944e5298989a3 |
| container_end_page | 439 |
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| container_start_page | 431 |
| container_title | Australasian plant pathology |
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| creator | Meitz, Julia C Linde, Celeste C Thompson, Alistair Langenhoven, Shaun McLeod, Adéle |
| description | The oomycete Phytophthora capsici is an economically important plant pathogen worldwide. Not much is known about this pathogen in South Africa. Therefore, the first aim of the study was to obtain a better understanding of the host range and distribution of the pathogen. Second, South African P. capsici isolates were characterised through mating-type tests and genotyping with eight microsatellite markers (simple sequence repeat markers (SSR)) that were developed from the draft genome sequence. Analyses of diseased samples from 2000 to 2008 showed that P. capsici was present in at least five provinces on cucurbitaceous ( Cucumis melo and Cucurbita spp.) and solanaceous ( Capsicum spp. and Lycopersicon esculentum ) crops. Mating-type analyses of 78 isolates collected from these hosts showed that both mating types were present in all the sampled production regions. The mating-type frequency in the total South African population did not deviate significantly from a 1 : 1 ratio. Microsatellite genotyping of the isolates revealed that genotypic diversity was high in all regional populations with 59 of 78 isolates representing unique SSR multilocus genotypes (MLG). The remaining 19 isolates consisted of eight MLG that each included two to three isolates with identical SSR alleles. These identical isolates were considered to be clones that were derived from asexual reproduction due to significant P SEX |
| doi_str_mv | 10.1071/AP09075 |
| format | article |
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Not much is known about this pathogen in South Africa. Therefore, the first aim of the study was to obtain a better understanding of the host range and distribution of the pathogen. Second, South African P. capsici isolates were characterised through mating-type tests and genotyping with eight microsatellite markers (simple sequence repeat markers (SSR)) that were developed from the draft genome sequence. Analyses of diseased samples from 2000 to 2008 showed that P. capsici was present in at least five provinces on cucurbitaceous ( Cucumis melo and Cucurbita spp.) and solanaceous ( Capsicum spp. and Lycopersicon esculentum ) crops. Mating-type analyses of 78 isolates collected from these hosts showed that both mating types were present in all the sampled production regions. The mating-type frequency in the total South African population did not deviate significantly from a 1 : 1 ratio. Microsatellite genotyping of the isolates revealed that genotypic diversity was high in all regional populations with 59 of 78 isolates representing unique SSR multilocus genotypes (MLG). The remaining 19 isolates consisted of eight MLG that each included two to three isolates with identical SSR alleles. These identical isolates were considered to be clones that were derived from asexual reproduction due to significant P SEX <0.003 and P SEX ( f ) <0.008 probability values. Clonal genotypes were identified within and between production regions (Limpopo and Mpumalanga), on different crops (bell pepper, piquanté pepper and tomato) and across years.</description><identifier>ISSN: 0815-3191</identifier><identifier>ISSN: 0156-0972</identifier><identifier>EISSN: 1448-6032</identifier><identifier>DOI: 10.1071/AP09075</identifier><language>eng</language><publisher>Dordrecht: Collingwood, Victoria: CSIRO Publishing</publisher><subject>Agriculture ; Asexual reproduction ; Biomedical and Life Sciences ; blight ; Capsicum ; Crops ; Cucumis melo ; Cucurbita ; Ecology ; Entomology ; Genetic diversity ; Genetic markers ; Genomes ; Genotypes ; Genotyping ; Host range ; Life Sciences ; Lycopersicon esculentum ; Mating types ; Microsatellites ; mortality ; Nucleotide sequence ; Oomycetes ; Pathogens ; Phytophthora capsici ; Plant Pathology ; Plant Sciences ; Sex ; Simple sequence repeats ; survival ; temperate climate ; temperate zones ; Vegetables</subject><ispartof>Australasian plant pathology, 2010-01, Vol.39 (5), p.431-439</ispartof><rights>Australasian Plant Pathology Society 2010</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c396t-140651efe1535ffaa98fde174a6bd2c6ab69b07665aac99a3323944e5298989a3</citedby><cites>FETCH-LOGICAL-c396t-140651efe1535ffaa98fde174a6bd2c6ab69b07665aac99a3323944e5298989a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids></links><search><creatorcontrib>Meitz, Julia C</creatorcontrib><creatorcontrib>Linde, Celeste C</creatorcontrib><creatorcontrib>Thompson, Alistair</creatorcontrib><creatorcontrib>Langenhoven, Shaun</creatorcontrib><creatorcontrib>McLeod, Adéle</creatorcontrib><title>Phytophthora capsici on vegetable hosts in South Africa: distribution, host range and genetic diversity</title><title>Australasian plant pathology</title><addtitle>Australasian Plant Pathol</addtitle><description>The oomycete Phytophthora capsici is an economically important plant pathogen worldwide. 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Microsatellite genotyping of the isolates revealed that genotypic diversity was high in all regional populations with 59 of 78 isolates representing unique SSR multilocus genotypes (MLG). The remaining 19 isolates consisted of eight MLG that each included two to three isolates with identical SSR alleles. These identical isolates were considered to be clones that were derived from asexual reproduction due to significant P SEX <0.003 and P SEX ( f ) <0.008 probability values. Clonal genotypes were identified within and between production regions (Limpopo and Mpumalanga), on different crops (bell pepper, piquanté pepper and tomato) and across years.</description><subject>Agriculture</subject><subject>Asexual reproduction</subject><subject>Biomedical and Life Sciences</subject><subject>blight</subject><subject>Capsicum</subject><subject>Crops</subject><subject>Cucumis melo</subject><subject>Cucurbita</subject><subject>Ecology</subject><subject>Entomology</subject><subject>Genetic diversity</subject><subject>Genetic markers</subject><subject>Genomes</subject><subject>Genotypes</subject><subject>Genotyping</subject><subject>Host range</subject><subject>Life Sciences</subject><subject>Lycopersicon esculentum</subject><subject>Mating types</subject><subject>Microsatellites</subject><subject>mortality</subject><subject>Nucleotide sequence</subject><subject>Oomycetes</subject><subject>Pathogens</subject><subject>Phytophthora capsici</subject><subject>Plant Pathology</subject><subject>Plant Sciences</subject><subject>Sex</subject><subject>Simple sequence repeats</subject><subject>survival</subject><subject>temperate climate</subject><subject>temperate zones</subject><subject>Vegetables</subject><issn>0815-3191</issn><issn>0156-0972</issn><issn>1448-6032</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><recordid>eNp90U1r3DAQBmBRWug2Lf0DgQhyaA91OmNZstXbEvoRCCSQ5mzGWtlW2FgbSQ7sv4-SbJuQQ9BhLg_vi2YY-4xwhFDj9-U5aKjlG7bAqmoKBaJ8yxbQoCwEanzPPsR4BYCVErBgw_m4TX4zptEH4oY20RnH_cRv7WATdWvLRx9T5G7iF35OI1_2wRn6wVcupuC6OTk_fXtAPNA0WE7Tig92ssmZjG5tiC5tP7J3Pa2j_bSbe-zy18-_x3-K07PfJ8fL08IIrVKBFSiJtrcohex7It30K4t1RapblUZRp3QHtVKSyGhNQpRCV5WVpW7yI7HHvjzmboK_mW1M7bWLxq7XNFk_x7aWEoUUtcjy66sSoYRG5h1Cpocv6JWfw5T_kRVKBQqlfKo2wccYbN9ugrumsM2ovT9NuzvNU3XMIq8sPM97SQ8eaU--pSG42F5elIACsKkllk0W-7va6IL_3_kv4A5bRZ9b</recordid><startdate>20100101</startdate><enddate>20100101</enddate><creator>Meitz, Julia C</creator><creator>Linde, Celeste C</creator><creator>Thompson, Alistair</creator><creator>Langenhoven, Shaun</creator><creator>McLeod, Adéle</creator><general>Collingwood, Victoria: CSIRO Publishing</general><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20100101</creationdate><title>Phytophthora capsici on vegetable hosts in South Africa: distribution, host range and genetic diversity</title><author>Meitz, Julia C ; Linde, Celeste C ; Thompson, Alistair ; Langenhoven, Shaun ; McLeod, Adéle</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c396t-140651efe1535ffaa98fde174a6bd2c6ab69b07665aac99a3323944e5298989a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Agriculture</topic><topic>Asexual reproduction</topic><topic>Biomedical and Life Sciences</topic><topic>blight</topic><topic>Capsicum</topic><topic>Crops</topic><topic>Cucumis melo</topic><topic>Cucurbita</topic><topic>Ecology</topic><topic>Entomology</topic><topic>Genetic diversity</topic><topic>Genetic markers</topic><topic>Genomes</topic><topic>Genotypes</topic><topic>Genotyping</topic><topic>Host range</topic><topic>Life Sciences</topic><topic>Lycopersicon esculentum</topic><topic>Mating types</topic><topic>Microsatellites</topic><topic>mortality</topic><topic>Nucleotide sequence</topic><topic>Oomycetes</topic><topic>Pathogens</topic><topic>Phytophthora capsici</topic><topic>Plant Pathology</topic><topic>Plant Sciences</topic><topic>Sex</topic><topic>Simple sequence repeats</topic><topic>survival</topic><topic>temperate climate</topic><topic>temperate zones</topic><topic>Vegetables</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Meitz, Julia C</creatorcontrib><creatorcontrib>Linde, Celeste C</creatorcontrib><creatorcontrib>Thompson, Alistair</creatorcontrib><creatorcontrib>Langenhoven, Shaun</creatorcontrib><creatorcontrib>McLeod, Adéle</creatorcontrib><collection>AGRIS</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Australasian plant pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Meitz, Julia C</au><au>Linde, Celeste C</au><au>Thompson, Alistair</au><au>Langenhoven, Shaun</au><au>McLeod, Adéle</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Phytophthora capsici on vegetable hosts in South Africa: distribution, host range and genetic diversity</atitle><jtitle>Australasian plant pathology</jtitle><stitle>Australasian Plant Pathol</stitle><date>2010-01-01</date><risdate>2010</risdate><volume>39</volume><issue>5</issue><spage>431</spage><epage>439</epage><pages>431-439</pages><issn>0815-3191</issn><issn>0156-0972</issn><eissn>1448-6032</eissn><abstract>The oomycete Phytophthora capsici is an economically important plant pathogen worldwide. Not much is known about this pathogen in South Africa. Therefore, the first aim of the study was to obtain a better understanding of the host range and distribution of the pathogen. Second, South African P. capsici isolates were characterised through mating-type tests and genotyping with eight microsatellite markers (simple sequence repeat markers (SSR)) that were developed from the draft genome sequence. Analyses of diseased samples from 2000 to 2008 showed that P. capsici was present in at least five provinces on cucurbitaceous ( Cucumis melo and Cucurbita spp.) and solanaceous ( Capsicum spp. and Lycopersicon esculentum ) crops. Mating-type analyses of 78 isolates collected from these hosts showed that both mating types were present in all the sampled production regions. The mating-type frequency in the total South African population did not deviate significantly from a 1 : 1 ratio. Microsatellite genotyping of the isolates revealed that genotypic diversity was high in all regional populations with 59 of 78 isolates representing unique SSR multilocus genotypes (MLG). The remaining 19 isolates consisted of eight MLG that each included two to three isolates with identical SSR alleles. These identical isolates were considered to be clones that were derived from asexual reproduction due to significant P SEX <0.003 and P SEX ( f ) <0.008 probability values. Clonal genotypes were identified within and between production regions (Limpopo and Mpumalanga), on different crops (bell pepper, piquanté pepper and tomato) and across years.</abstract><cop>Dordrecht</cop><pub>Collingwood, Victoria: CSIRO Publishing</pub><doi>10.1071/AP09075</doi><tpages>9</tpages></addata></record> |
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| ispartof | Australasian plant pathology, 2010-01, Vol.39 (5), p.431-439 |
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| subjects | Agriculture Asexual reproduction Biomedical and Life Sciences blight Capsicum Crops Cucumis melo Cucurbita Ecology Entomology Genetic diversity Genetic markers Genomes Genotypes Genotyping Host range Life Sciences Lycopersicon esculentum Mating types Microsatellites mortality Nucleotide sequence Oomycetes Pathogens Phytophthora capsici Plant Pathology Plant Sciences Sex Simple sequence repeats survival temperate climate temperate zones Vegetables |
| title | Phytophthora capsici on vegetable hosts in South Africa: distribution, host range and genetic diversity |
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