Cross-Sectional Analysis of the Polysaccharide Composition in Cellulosic Fiber Materials by Enzymatic Peeling/High-Performance Capillary Zone Electrophoresis

A combined enzymatic, chemical, and analytical approach was used to determine the cross-sectional carbohydrate composition in cellulosic fibers. The outer surface of cellulosic fibers was enzymatically removed layer-by-layer with precise quantitative control, and the monosaccharides in the peelings...

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Bibliographic Details
Published in:Biomacromolecules 2005-11, Vol.6 (6), p.3146-3151
Main Authors: Sjöberg, John, Potthast, Antje, Rosenau, Thomas, Kosma, Paul, Sixta, Herbert
Format: Article
Language:English
Subjects:
Applied sciences
Biocompatible Materials - chemistry
Carbohydrates
Carbohydrates - chemistry
Cellulose - chemistry
Chromatography
Chromatography, High Pressure Liquid
Composition
Electrophoresis
Electrophoresis, Capillary - methods
Enzymes
Exact sciences and technology
Fibers and threads
Forms of application and semi-finished materials
Hydrolysis
Kraft pulp
Macromolecular Substances - chemistry
Mixtures
Plant Structures - chemistry
Polymer industry, paints, wood
Polysaccharides
Polysaccharides - chemistry
Sulfites - chemistry
Technology of polymers
Textile fibers
Time Factors
Ultraviolet Rays
Xylans - chemistry
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Summary:A combined enzymatic, chemical, and analytical approach was used to determine the cross-sectional carbohydrate composition in cellulosic fibers. The outer surface of cellulosic fibers was enzymatically removed layer-by-layer with precise quantitative control, and the monosaccharides in the peelings were subsequently analyzed by high-performance capillary electrophoresis (HPCE) after precolumn derivatization with a UV label. This method was applied to dissolving pulps and regenerated cellulose fibers, with special emphasis on the cross-sectional distribution of hemicelluloses. Commercially available enzyme solutions were used, resulting in a reproducible peeling. Significant differences were found in the hemicellulose distribution across the fiber of different dissolving pulps, dependent on both natural source (beech or spruce) and preparation process (acidic sulfite cook or prehydrolysis kraft cook). Among the dissolving pulps, beech prehydrolysis kraft pulp showed the highest enrichment of surface xylan. Similar, albeit smaller, differences were noticed between various regenerated fibers (viscose, viscose Modal, and Lyocell):  a thin hemicellulose-rich outermost layer was found in all the regenerated fibers studied.
ISSN:1525-7797
1526-4602
1526-4602
DOI:10.1021/bm050471j