Immunolocalization of β-(1–4)-D-galactan, xyloglucans and xylans in the reaction xylem fibres of Leucaena leucocephala (Lam.) de Wit

• Published in: Plant physiology and biochemistry 2019-09, Vol.142, p.217-223
• Main Authors: Pramod, S., Rajput, Kishore S., Rao, Karumanchi S.
• Format: Article
• Language: English
• Subjects: Immuno electron microscopy; Immuno electron microscopyβ-(1–4)-D-galactan; Leucaena leucocephala; Reaction xylem; Xylan; Xyloglucan; β-(1–4)-D-galactan
• ISSN: 0981-9428; 1873-2690; 1873-2690
• DOI: 10.1016/j.plaphy.2019.07.013

Cell wall architecture of tension wood fibres represents a suitable biological system to study the mechanism of growth and maintenance of posture of trees growing under various physical and physiological growth constraints. In the present study, we investigated the spatial distributions of β-(1–4)-D-galactan, xyloglucan and xylans (both less and highly substituted) in the opposite and tension wood fibres of bent Leucaena leucocephala by immunolabelling with monoclonal antibodies LM5, CCRCM1, LM10 and LM11 specific to these carbohydrate epitopes. The presence of non-lignified, tertiary wall layer is the typical tension wood characteristic associated with the reaction xylem fibres in Leucaena. LM5 labelling of opposite fibres showed weak labelling in the cell walls indicating less concentration of β-(1–4)-D-galactans while tension wood showed strong labelling in the tertiary wall layer suggesting the gelatinous layer (G-layer) has a strong cross linking with β-(1–4)-D-galactans. Xyloglucan distribution was more in the compound middle lamellae and the primary wall-S1 layer boundary of tension wood fibres as compared to that of opposite wood. A weak labelling was also evident near the boundary between the G-layer and the secondary wall of tension wood fibres. The secondary wall of opposite and tension wood fibres showed a strong distribution of both ls ACG Xs (LM10) and hs ACG Xs (LM11) while a weak labelling was noticed in the compound middle lamella. Tension wood fibres also showed strong xylan labelling mainly confined to the lignified secondary walls while the G-layer showed weak xylan labelling. In conclusion, our results suggest that β-(1–4)-D-galactans and xyloglucans could be implicated in the tensile stress generation within the G-layer of tension wood fibres of Leucaena leucocephala. •Immuno-localization of cell wall polymers.•Variation in cell wall topochemistry of tension wood and normal wood fibres in L. leucocephala.•Gelatinous layer showed distinct variation in distribution pattern of β-(1–4)-D-galactans and xylans.•Secondary wall lignification and xylan distribution may be related.•Probable role of β-(1–4)-D-galactans, xyloglucans and xylans in tensile stress generation.