Covalent VEGF protein immobilization on resorbable polymeric surfaces

Vascular endothelial growth factor type protein (VEGF), a potent angiogenic effector molecule, was successfully covalently immobilized onto the surfaces of the resorbable polymers poly(L‐lactic acid) (PLLA) and poly(ε‐caprolactone) (PCL) through a three‐step strategy. The surfaces were first covalen...

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Bibliographic Details
Published in:Polymers for advanced technologies 2011-12, Vol.22 (12), p.2368-2373
Main Authors: Edlund, U., Sauter, T., Albertsson, A.-C.
Format: Article
Language:English
Subjects:
Covalence
ELISA
Grafting
poly(epsilon-caprolactone)
poly(ε-caprolactone)
polylactide
Polymers
Proteins
Spacers
surface
Topography
vapor phase grafting
VEGF
X-ray photoelectron spectroscopy
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Summary:Vascular endothelial growth factor type protein (VEGF), a potent angiogenic effector molecule, was successfully covalently immobilized onto the surfaces of the resorbable polymers poly(L‐lactic acid) (PLLA) and poly(ε‐caprolactone) (PCL) through a three‐step strategy. The surfaces were first covalently grafted with poly(acrylic acid) using non‐destructive and solvent free vapor‐phase grafting. A diamine spacer was coupled to the carboxylic acid pendant groups on the graft chains using EDC/NHS chemistry and VEGF was finally covalently attached to the amine linkers. The chemistry and topography of the modified substrates were quantitatively and qualitatively verified with XPS, ATR‐FTIR, UV–VIS, SEM, and ELISA. Copyright © 2010 John Wiley & Sons, Ltd.
ISSN:1042-7147
1099-1581
1099-1581
DOI:10.1002/pat.1772