Coagulase-negative staphylococci isolated from sternal wound infections after cardiac surgery: attachment to and accumulation on sternal fixation stainless steel wires

Sternal wound infection (SWI) is a serious complication after cardiac surgery. Coagulase‐negative staphylococci (CoNS) have been found to be the most common pathogen involved in this postoperative infection related to implanted foreign materials, i.e. sternal fixation wires made from stainless steel...

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Bibliographic Details
Published in:APMIS : acta pathologica, microbiologica et immunologica Scandinavica microbiologica et immunologica Scandinavica, 2007-02, Vol.115 (2), p.142-151
Main Authors: OLSSON, EMMA, FRIBERG, ÖRJAN, VENIZELOS, NIKOLAOS, KOSKELA, ANITA, KÄLLMAN, JAN, SÖDERQUIST, BO
Format: Article
Language:English
Subjects:
Bacterial Adhesion
Bacteriology
biofilm
Biofilms
Biological and medical sciences
Cardiac Surgical Procedures
Coagulase - analysis
Fundamental and applied biological sciences. Psychology
Humans
in vitro test
Infectious diseases
Internal Fixators
Kirurgi
Kirurgisk forskning
Medical sciences
MEDICIN
MEDICINE
Microbiology
Microbiology, immunology, infectious diseases
Mikrobiologi, immunologi, infektionssjukdomar
Miscellaneous
Operon
Phenotype
Phylogeny
stainless steel
staphylococcal infections
Staphylococcus - classification
Staphylococcus - genetics
Staphylococcus - growth & development
Staphylococcus - isolation & purification
Sternum - microbiology
Surgery
Surgical research
Surgical Wound Infection - microbiology
Thoracic surgery
Thoraxkirurgi
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Description
Summary:Sternal wound infection (SWI) is a serious complication after cardiac surgery. Coagulase‐negative staphylococci (CoNS) have been found to be the most common pathogen involved in this postoperative infection related to implanted foreign materials, i.e. sternal fixation wires made from stainless steel. In this study a rapid and simple assay was developed for studying attachment and accumulation of CoNS on stainless steel wires in vitro using [3H] thymidine. The method showed a potential to detect differences in the dynamics of the adherence patterns among various CoNS isolates. However, no differences in attachment and accumulation were found between isolates causing deep SWI after cardiac surgery and contaminant isolates. In addition, there were no differences in the distribution of the ica operon between the two groups, as determined by polymerase chain reaction (PCR). Nevertheless, the ability to produce biofilm was found to be present significantly more frequently among SWI isolates than among contaminants.
ISSN:0903-4641
1600-0463
1600-0463
DOI:10.1111/j.1600-0463.2007.apm_559.x