Neisseria gonorrhoeae Sequence Typing for Antimicrobial Resistance, a Novel Antimicrobial Resistance Multilocus Typing Scheme for Tracking Global Dissemination of N. gonorrhoeae Strains

• Published in: Journal of clinical microbiology 2017-05, Vol.55 (5), p.1454-1468
• Main Authors: Demczuk, W, Sidhu, S, Unemo, M, Whiley, D M, Allen, V G, Dillon, J R, Cole, M, Seah, C, Trembizki, E, Trees, D L, Kersh, E N, Abrams, A J, de Vries, H J C, van Dam, A P, Medina, I, Bharat, A, Mulvey, M R, Van Domselaar, G, Martin, I
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Anti-Bacterial Agents - pharmacology; antimicrobial resistance; Azithromycin - pharmacology; Bacteriology; Cephalosporins - pharmacology; Drug Resistance, Multiple, Bacterial - genetics; Fluoroquinolones - pharmacology; Gonorrhea - epidemiology; Gonorrhea - microbiology; Humans; molecular epidemiology; Multilocus Sequence Typing - methods; Neisseria gonorrhoeae; Neisseria gonorrhoeae - classification; Neisseria gonorrhoeae - drug effects; Neisseria gonorrhoeae - genetics; Neisseria gonorrhoeae - isolation & purification; sequence typing
• ISSN: 0095-1137; 1098-660X; 1098-660X
• DOI: 10.1128/jcm.00100-17

A curated Web-based user-friendly sequence typing tool based on antimicrobial resistance determinants in was developed and is publicly accessible (https://ngstar.canada.ca). The Sequence Typing for Antimicrobial Resistance (NG-STAR) molecular typing scheme uses the DNA sequences of 7 genes ( , , , , , , and 23S rRNA) associated with resistance to β-lactam antimicrobials, macrolides, or fluoroquinolones. NG-STAR uses the entire sequence, combining the historical nomenclature for types I to XXXVIII with novel nucleotide sequence designations; the full sequence and a portion of its promoter region; portions of , , , and ; and 23S rRNA sequences. NG-STAR grouped 768 isolates into 139 sequence types (STs) ( = 660) consisting of 29 clonal complexes (CCs) having a maximum of a single-locus variation, and 76 NG-STAR STs ( = 109) were identified as unrelated singletons. NG-STAR had a high Simpson's diversity index value of 96.5% (95% confidence interval [CI] = 0.959 to 0.969). The most common STs were NG-STAR ST-90 ( = 100; 13.0%), ST-42 and ST-91 ( = 45; 5.9%), ST-64 ( = 44; 5.72%), and ST-139 ( = 42; 5.5%). Decreased susceptibility to azithromycin was associated with NG-STAR ST-58, ST-61, ST-64, ST-79, ST-91, and ST-139 ( = 156; 92.3%); decreased susceptibility to cephalosporins was associated with NG-STAR ST-90, ST-91, and ST-97 ( = 162; 94.2%); and ciprofloxacin resistance was associated with NG-STAR ST-26, ST-90, ST-91, ST-97, ST-150, and ST-158 ( = 196; 98.0%). All isolates of NG-STAR ST-42, ST-43, ST-63, ST-81, and ST-160 ( = 106) were susceptible to all four antimicrobials. The standardization of nomenclature associated with antimicrobial resistance determinants through an internationally available database will facilitate the monitoring of the global dissemination of antimicrobial-resistant strains.