Chemical microenvironments and single-cell carbon and nitrogen uptake in field-collected colonies of Trichodesmium under different pCO(2)

• Published in: The ISME Journal 2017, Vol.11 (6), p.1305
• Main Authors: Eichner, Meri J., Klawonn, Isabell, Wilson, Samuel T., Littmann, Sten, Whitehouse, Martin J., Church, Matthew J., Kuypers, Marcel M. M., Karl, David M., Ploug, Helle
• Format: Article
• Language: English
• ISSN: 1751-7370; 1751-7362
• DOI: 10.1038/ismej.2017.15

Gradients of oxygen (O-2) and pH, as well as small-scale fluxes of carbon (C), nitrogen (N) and O-2 were investigated under different partial pressures of carbon dioxide (pCO(2)) in field-collected colonies of the marine dinitrogen (N-2)-fixing cyanobacterium Trichodesmium. Microsensor measurements indicated that cells within colonies experienced large fluctuations in O-2, pH and CO2 concentrations over a day-night cycle. O-2 concentrations varied with light intensity and time of day, yet colonies exposed to light were supersaturated with O-2 (up to similar to 200%) throughout the light period and anoxia was not detected. Alternating between light and dark conditions caused a variation in pH levels by on average 0.5 units (equivalent to 15 nmol l(-1) proton concentration). Single-cell analyses of C and N assimilation using secondary ion mass spectrometry (SIMS; large geometry SIMS and nanoscale SIMS) revealed high variability in metabolic activity of single cells and trichomes of Trichodesmium, and indicated transfer of C and N to colony-associated non-photosynthetic bacteria. Neither O-2 fluxes nor C fixation by Trichodesmium were significantly influenced by short-term incubations under different pCO(2) levels, whereas N-2 fixation increased with increasing pCO(2). The large range of metabolic rates observed at the single-cell level may reflect a response by colony-forming microbial populations to highly variable microenvironments.