A Classical Brown Adipose Tissue mRNA Signature Partly Overlaps with Brite in the Supraclavicular Region of Adult Humans

Human brown adipose tissue (BAT) has been detected in adults but was recently suggested to be of brite/beige origin. We collected BAT from the supraclavicular region in 21 patients undergoing surgery for suspected cancer in the neck area and assessed the gene expression of established murine markers...

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Bibliographic Details
Published in:Cell metabolism 2013-05, Vol.17 (5), p.798-805
Main Authors: Jespersen, Naja Zenius, Larsen, Therese Juhlin, Peijs, Lone, Daugaard, Søren, Homøe, Preben, Loft, Annika, de Jong, Jasper, Mathur, Neha, Cannon, Barbara, Nedergaard, Jan, Pedersen, Bente Klarlund, Møller, Kirsten, Scheele, Camilla
Format: Article
Language:English
Subjects:
Adipocytes - metabolism
Adipocytes, White - metabolism
Adipose Tissue, Brown - metabolism
Adipose Tissue, White - metabolism
Adult
Aged
Aged, 80 and over
Animals
Biomarkers - metabolism
Cell Differentiation - genetics
Down-Regulation
Female
fysiologi
Humans
Ion Channels - genetics
Ion Channels - metabolism
Male
Middle Aged
Mitochondrial Proteins - genetics
Mitochondrial Proteins - metabolism
Norepinephrine - metabolism
Physiology
RNA, Messenger - genetics
RNA, Messenger - metabolism
Transcriptome
Uncoupling Protein 1
Up-Regulation
Young Adult
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Summary:Human brown adipose tissue (BAT) has been detected in adults but was recently suggested to be of brite/beige origin. We collected BAT from the supraclavicular region in 21 patients undergoing surgery for suspected cancer in the neck area and assessed the gene expression of established murine markers for brown, brite/beige, and white adipocytes. We demonstrate that a classical brown expression signature, including upregulation of miR-206, miR-133b, LHX8, and ZIC1 and downregulation of HOXC8 and HOXC9, coexists with an upregulation of two newly established brite/beige markers, TBX1 and TMEM26. A similar mRNA expression profile was observed when comparing isolated human adipocytes from BAT and white adipose tissue (WAT) depots, differentiated in vitro. In conclusion, our data suggest that human BAT might consist of both classical brown and recruitable brite adipocytes, an observation important for future considerations on how to induce human BAT. •Classical brown adipose tissue is present in adult humans•Brite precursor markers were upregulated along with classical brown markers•The BAT signature observed in tissue was generally conserved in isolated cells
ISSN:1550-4131
1932-7420
1932-7420
DOI:10.1016/j.cmet.2013.04.011