Selection of candidate coding DNA barcoding regions for use on land plants

An in silico screen of 41 of the 81 coding regions of the Nicotiana plastid genome generated a shortlist of 12 candidates as DNA barcoding loci for land plants. These loci were evaluated for amplification and sequence variation against a reference set of 98 land plant taxa. The deployment of multipl...

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Published in:Botanical journal of the Linnean Society 2009, Vol.159 (1), p.1-11
Main Authors: FORD, CAROLINE S, AYRES, KAREN L, TOOMEY, NICOLA, HAIDER, NADIA, VAN ALPHEN STAHL, JONATHAN, KELLY, LAURA J, WIKSTRÖM, NIKLAS, HOLLINGSWORTH, PETER M, DUFF, R. JOEL, HOOT, SARAH B, COWAN, ROBYN S, CHASE, MARK W, WILKINSON, MIKE J
Format: Article
Language:English
Subjects:
Biologi
Biology
genetic barcodes
matK
MT-PCR
multi-locus barcode
NATURAL SCIENCES
NATURVETENSKAP
Nicotiana
plant identification
plant taxonomy
plastid genes
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Summary:An in silico screen of 41 of the 81 coding regions of the Nicotiana plastid genome generated a shortlist of 12 candidates as DNA barcoding loci for land plants. These loci were evaluated for amplification and sequence variation against a reference set of 98 land plant taxa. The deployment of multiple primers and a modified multiplexed tandem polymerase chain reaction yielded 85-94% amplification across taxa, and mean sequence differences between sister taxa of 6.1 from 156 bases of accD to 22 from 493 bases of matK. We conclude that loci should be combined for effective diagnosis, and recommend further investigation of the following six loci: matK, rpoB, rpoC1, ndhJ, ycf5 and accD.
ISSN:0024-4074
1095-8339
1095-8339
DOI:10.1111/j.1095-8339.2008.00938.x