A 54-kDa Fragment of the Poly(A)-specific Ribonuclease Is an Oligomeric, Processive, and Cap-interacting Poly(A)-specific 3′ Exonuclease

• Published in: The Journal of biological chemistry 2000-08, Vol.275 (31), p.24222-24230
• Main Authors: Martı́nez, Javier, Ren, Yan-Guo, Thuresson, Ann-Charlotte, Hellman, Ulf, Åström, Jonas, Virtanen, Anders
• Format: Article
• Language: English
• Subjects: 3' exonuclease; Adenosine Monophosphate - metabolism; Animals; Cattle; Chromatography, Affinity - methods; Exoribonucleases - isolation & purification; Exoribonucleases - metabolism; Exoribonucleases/isolation & purification/metabolism; Movement; Peptide Fragments - metabolism; Protein Binding; Protein Structure, Quaternary; ribonuclease; RNA Caps - metabolism; RNA Processing, Post-Transcriptional; Substrate Specificity; Thymus Gland - enzymology
• ISSN: 0021-9258; 1083-351X; 1083-351X
• DOI: 10.1074/jbc.M001705200

We have previously identified a HeLa cell 3′ exonuclease specific for degrading poly(A) tails of mRNAs. Here we report on the purification and identification of a calf thymus 54-kDa polypeptide associated with a similar 3′ exonuclease activity. The 54-kDa polypeptide was shown to be a fragment of the poly(A)-specific ribonuclease 74-kDa polypeptide. The native molecular mass of the nuclease activity was estimated to be 180–220 kDa. Protein/protein cross-linking revealed an oligomeric structure, most likely consisting of three subunits. The purified nuclease activity released 5′-AMP as the reaction product and degraded poly(A) in a highly processive fashion. The activity required monovalent cations and was dependent on divalent metal ions. The RNA substrate requirement was investigated, and it was found that the nuclease was highly poly(A)-specific and that only 3′ end-located poly(A) was degraded by the activity. RNA substrates capped with m7G(5′)ppp(5′)G were more efficiently degraded than noncapped RNA substrates. Addition of free m7G(5′)ppp(5′)G cap analogue inhibited poly(A) degradationin vitro, suggesting a functional link between the RNA 5′ end cap structure and poly(A) degradation at the 3′ end of the RNA.