Evaluation of Different Cleaning Strategies for Removal of Contaminating DNA Molecules

Decontamination strategies and their efficiencies are crucial when performing routine forensic analysis, and many factors influence the choice of agent to use. In this study, the effects of ten different cleaning strategies were evaluated to compare their ability to remove contaminating DNA molecule...

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Bibliographic Details
Published in:Genes 2022-01, Vol.13 (1), p.162
Main Authors: Nilsson, Martina, De Maeyer, Hanne, Allen, Marie
Format: Article
Language:English
Subjects:
Blood cells
Cleaning
cleaning strategies
Crime scenes
Decontamination
Decontamination - methods
Deoxyribonucleic acid
DNA
DNA - blood
DNA Contamination
DNA molecules
Efficiency
Ethanol
Forensic science
Households
Humans
Laboratories
Male
Mitochondrial DNA
Sodium
Sodium hypochlorite
Specimen Handling - methods
Specimen Handling - standards
Ultraviolet radiation
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Summary:Decontamination strategies and their efficiencies are crucial when performing routine forensic analysis, and many factors influence the choice of agent to use. In this study, the effects of ten different cleaning strategies were evaluated to compare their ability to remove contaminating DNA molecules. Cell-free DNA or blood was deposited on three surfaces (plastic, metal, and wood) and decontaminated with various treatments. The quantities of recovered DNA, obtained by swabbing the surfaces after cleaning using the different strategies, was analyzed by real-time PCR. Large differences in the DNA removal efficiencies were observed between different cleaning strategies, as well as between different surfaces. The most efficient cleaning strategies for cell-free DNA were the different sodium hypochlorite solutions and Trigene , for which a maximum of 0.3% DNA was recovered on all three surfaces. For blood, a maximum of 0.8% of the deposited DNA was recovered after using Virkon for decontamination. The recoveries after using these cleaning strategies correspond to DNA from only a few cells, out of 60 ng of cell-free DNA or thousands of deposited blood cells.
ISSN:2073-4425
2073-4425
DOI:10.3390/genes13010162