Evaluation of Different Cleaning Strategies for Removal of Contaminating DNA Molecules

• Published in: Genes 2022-01, Vol.13 (1), p.162
• Main Authors: Nilsson, Martina, De Maeyer, Hanne, Allen, Marie
• Format: Article
• Language: English
• Subjects: Blood cells; Cleaning; cleaning strategies; Crime scenes; Decontamination; Decontamination - methods; Deoxyribonucleic acid; DNA; DNA - blood; DNA Contamination; DNA molecules; Efficiency; Ethanol; Forensic science; Households; Humans; Laboratories; Male; Mitochondrial DNA; Sodium; Sodium hypochlorite; Specimen Handling - methods; Specimen Handling - standards; Ultraviolet radiation
• ISSN: 2073-4425; 2073-4425
• DOI: 10.3390/genes13010162

Decontamination strategies and their efficiencies are crucial when performing routine forensic analysis, and many factors influence the choice of agent to use. In this study, the effects of ten different cleaning strategies were evaluated to compare their ability to remove contaminating DNA molecules. Cell-free DNA or blood was deposited on three surfaces (plastic, metal, and wood) and decontaminated with various treatments. The quantities of recovered DNA, obtained by swabbing the surfaces after cleaning using the different strategies, was analyzed by real-time PCR. Large differences in the DNA removal efficiencies were observed between different cleaning strategies, as well as between different surfaces. The most efficient cleaning strategies for cell-free DNA were the different sodium hypochlorite solutions and Trigene , for which a maximum of 0.3% DNA was recovered on all three surfaces. For blood, a maximum of 0.8% of the deposited DNA was recovered after using Virkon for decontamination. The recoveries after using these cleaning strategies correspond to DNA from only a few cells, out of 60 ng of cell-free DNA or thousands of deposited blood cells.