On the applicability of high frequency acoustic shear mode biosensing in view of thickness limitations set by the film resonance

The IC-compatible thin film bulk acoustic resonator (FBAR) technology has made it possible to move the thickness excited shear mode sensing of biological layers into a new sensing regime using substantially higher operation frequencies than the conventionally used quartz crystal microbalance (QCM)....

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Bibliographic Details
Published in:Biosensors & bioelectronics 2009-07, Vol.24 (11), p.3387-3390
Main Authors: Wingqvist, G., Anderson, H., Lennartsson, C., Weissbach, T., Yantchev, V., Lloyd Spetz, A.
Format: Article
Language:English
Subjects:
Acoustics - instrumentation
Biological and medical sciences
Biosensing Techniques - instrumentation
Biosensors
Biotechnology
Electronics
Elektronik
Equipment Design
Equipment Failure Analysis
Fibrinogen
Fundamental and applied biological sciences. Psychology
High frequency
Membranes, Artificial
Methods. Procedures. Technologies
NATURAL SCIENCES
NATURVETENSKAP
Protein Array Analysis - instrumentation
Proteins - analysis
Proteins - chemistry
Shear mode electroacoustic sensing
Shear Strength
Streptavidin–biotinated BSA
TECHNOLOGY
TEKNIKVETENSKAP
Thin film bulk acoustic resonator (FBAR)
Various methods and equipments
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Summary:The IC-compatible thin film bulk acoustic resonator (FBAR) technology has made it possible to move the thickness excited shear mode sensing of biological layers into a new sensing regime using substantially higher operation frequencies than the conventionally used quartz crystal microbalance (QCM). The limitations of the linear range set by the film resonance using viscoelastic protein films are here for the first time addressed specifically for FBARs operating at 700 MHz up to 1.5 GHz. Two types of protein multilayer sensing were employed; one utilizing alternating layers of streptavidin and biotinated BSA and the other using stepwise cross-linking of fibrinogen with EDC/NHS activation of its carboxyl groups. In both cases the number of protein layers within the linear regime is well above the number of protein layers usually used in biosensor applications, further verifying the applicability of the FBAR as a biosensor. Theoretical calculations are also presented using well established physical models to illustrate the expected behavior of the FBAR sensor, in view of both the frequency and the dissipation shifts.
ISSN:0956-5663
1873-4235
1873-4235
DOI:10.1016/j.bios.2009.04.021