Liquid chromatography-tandem mass spectrometry-based fragmentation analysis of glycopeptides

The use of liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS n ) for the glycoproteomic characterization of glycopeptides is a growing field of research. The N- and O-glycosylated peptides (N- and O-glycopeptides) analyzed typically originate from protease-digested gl...

Full description

Saved in:
Bibliographic Details
Published in:Glycoconjugate journal 2016-06, Vol.33 (3), p.261-272
Main Author: Nilsson, Jonas
Format: Article
Language:English
Subjects:
Biochemistry
Biomedical and Life Sciences
c-trap dissociation
Chromatography, Liquid - methods
Clinical Medicine
collision-induced
Collision-induced dissociation
dissociation
Electron capture dissociation
Electron transfer dissociation
glycan structure
Glycopeptides
Glycopeptides - chemistry
Glycoproteins
Glycoproteomics
Higher-energy collisional dissociation
human cerebrospinal-fluid
Klinisk medicin
lc-ms/ms
Life Sciences
Mass spectrometry
n-glycosylation analysis
o-glycosylation
Oxonium ions
Pathology
Peptides
protein glycosylation
Proteomics
Review
site-specific characterization
Tandem Mass Spectrometry - methods
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The use of liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS n ) for the glycoproteomic characterization of glycopeptides is a growing field of research. The N- and O-glycosylated peptides (N- and O-glycopeptides) analyzed typically originate from protease-digested glycoproteins where many of them are expected to be biomedically important. Examples of LC-MS 2 and MS 3 fragmentation strategies used to pursue glycan structure, peptide identity and attachment-site identification analyses of glycopeptides are described in this review. MS 2 spectra, using the CID and HCD fragmentation techniques of a complex biantennary N-glycopeptide and a core 1 O-glycopeptide, representing two examples of commonly studied glycopeptide types, are presented. A few practical tips for accomplishing glycopeptide analysis using reversed-phase LC-MS n shotgun proteomics settings, together with references to the latest glycoproteomic studies, are presented.
ISSN:0282-0080
1573-4986
DOI:10.1007/s10719-016-9649-3