Evidence for different exocytosis pathways in dendritic and terminal dopamine release in vivo

Although dendritic release was first proposed in the 1970s, the mechanism of release is still subject to debate. We have used in vivo microdialysis to study the acute effects of botulinum toxin A, B and tetanus toxin injected in the substantia nigra or striatum of freely moving rats. Spontaneous and...

Full description

Saved in:
Bibliographic Details
Published in:Brain research 2002-09, Vol.950 (1), p.245-253
Main Authors: Bergquist, Filip, Niazi, Haydeh Shahabi, Nissbrandt, Hans
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Botulinum Toxins
Botulinum Toxins - pharmacology
Botulinum Toxins, Type A
Central nervous system
Central neurotransmission. Neuromudulation. Pathways and receptors
Corpus Striatum
Corpus Striatum - drug effects
Corpus Striatum - metabolism
Dendrites
Dendrites - drug effects
Dendrites - metabolism
Dopamine
Dopamine - metabolism
Dopamine release
drug effects
Exocytosis
Exocytosis - drug effects
Exocytosis - physiology
Fundamental and applied biological sciences. Psychology
Fysiologi
Male
metabolism
Neural Pathways
Neural Pathways - drug effects
Neural Pathways - metabolism
pharmacology
Physiology
Presynaptic Terminals
Presynaptic Terminals - drug effects
Presynaptic Terminals - metabolism
Rats
Rats, Sprague-Dawley
secretion
SNAP-25
Sprague-Dawley
Striatum
Substantia Nigra
Substantia Nigra - drug effects
Substantia Nigra - metabolism
Synaptobrevin
Tetanus Toxin
Tetanus Toxin - pharmacology
Vertebrates: nervous system and sense organs
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Although dendritic release was first proposed in the 1970s, the mechanism of release is still subject to debate. We have used in vivo microdialysis to study the acute effects of botulinum toxin A, B and tetanus toxin injected in the substantia nigra or striatum of freely moving rats. Spontaneous and evoked dopamine release decreased in both regions after treatment with the SNAP-25 (synaptosome-associated protein of 25 kDa) cleaving protease botulinum toxin A (1000 mouse lethal doses, MLD). Tetanus toxin (4000 MLD) did not significantly change spontaneous or evoked dopamine release in striatum or in the substantia nigra. Another synaptobrevin cleaving protease, botulinum toxin B, inhibited release in the striatum by 55% but did not affect dopamine release when injected in the substantia nigra. The results indicate that both terminal and somatodendritic dopamine release need intact SNAP-25 to occur, but somatodendritic dopamine release in contrast to terminal release depends on a botulinum toxin B resistant pathway.
ISSN:0006-8993
1872-6240
DOI:10.1016/S0006-8993(02)03047-0