Characterization of the Plasmodium Interspersed Repeats (PIR) proteins of Plasmodium chabaudi indicates functional diversity

Plasmodium multigene families play a central role in the pathogenesis of malaria. The Plasmodium interspersed repeat ( pir ) genes comprise the largest multigene family in many Plasmodium spp. However their function(s) remains unknown. Using the rodent model of malaria, Plasmodium chabaudi , we show...

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Bibliographic Details
Published in:Scientific reports 2016-03, Vol.6 (1), p.23449-23449, Article 23449
Main Authors: Yam, Xue Yan, Brugat, Thibaut, Siau, Anthony, Lawton, Jennifer, Wong, Daniel S., Farah, Abdirahman, Twang, Jing Shun, Gao, Xiaohong, Langhorne, Jean, Preiser, Peter R.
Format: Article
Language:English
Subjects:
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Animals
Blood
Erythrocytes
Erythrocytes - parasitology
Genes, Protozoan
Humanities and Social Sciences
Interspersed Repetitive Sequences
Localization
Malaria
Malaria - genetics
Malaria - parasitology
Medicin och hälsovetenskap
Merozoites
Mice
multidisciplinary
Multigene Family
Plasmodium chabaudi - genetics
Plasmodium chabaudi - pathogenicity
Protozoan Proteins - biosynthesis
Protozoan Proteins - physiology
Rosette formation
Science
Vector-borne diseases
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Summary:Plasmodium multigene families play a central role in the pathogenesis of malaria. The Plasmodium interspersed repeat ( pir ) genes comprise the largest multigene family in many Plasmodium spp. However their function(s) remains unknown. Using the rodent model of malaria, Plasmodium chabaudi , we show that individual CIR proteins have differential localizations within infected red cell (iRBC), suggesting different functional roles in a blood-stage infection. Some CIRs appear to be located on the surface of iRBC and merozoites and are therefore well placed to interact with host molecules. In line with this hypothesis, we show for the first time that a subset of recombinant CIRs bind mouse RBCs suggesting a role for CIR in rosette formation and/or invasion. Together, our results unravel differences in subcellular localization and ability to bind mouse erythrocytes between the members of the cir family, which strongly suggest different functional roles in a blood-stage infection.
ISSN:2045-2322
2045-2322
DOI:10.1038/srep23449