Activation of mTORC1 by leucine is potentiated by branched-chain amino acids and even more so by essential amino acids following resistance exercise

Protein synthesis is stimulated by resistance exercise and intake of amino acids, in particular leucine. Moreover, activation of mammalian target of rapamycin complex 1 (mTORC1) signaling by leucine is potentiated by the presence of other essential amino acids (EAA). However, the contribution of the...

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Bibliographic Details
Published in:American Journal of Physiology: Cell Physiology 2016-06, Vol.310 (11), p.C874-C884
Main Authors: Moberg, Marcus, Apró, William, Ekblom, Björn, van Hall, Gerrit, Holmberg, Hans-Christer, Blomstrand, Eva
Format: Article
Language:English
Subjects:
4E-BP1
Adaptor Proteins, Signal Transducing - metabolism
Adult
Amino acids
Amino Acids, Branched-Chain - administration & dosage
Amino Acids, Essential - administration & dosage
BCAA
Biopsy
Blood Glucose - metabolism
Energy Metabolism - drug effects
Eukaryotic Initiation Factor-4E - metabolism
Exercise
Healthy Volunteers
Humans
Insulin - blood
Lactic Acid - blood
Leucine - administration & dosage
Male
Mechanistic Target of Rapamycin Complex 1
Medicin och hälsovetenskap
Medicin/Teknik
Medicine/Technology
Multiprotein Complexes - agonists
Multiprotein Complexes - metabolism
Muscle, Skeletal - drug effects
Muscle, Skeletal - enzymology
p70S6 kinase 1
Phosphoproteins - metabolism
Phosphorylation
Protein Biosynthesis - drug effects
Protein synthesis
Resistance Training
Ribosomal Protein S6 Kinases, 70-kDa - metabolism
S6K1
Signal Transduction - drug effects
Skeletal muscle
Time Factors
TOR Serine-Threonine Kinases - metabolism
Treatment Outcome
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Summary:Protein synthesis is stimulated by resistance exercise and intake of amino acids, in particular leucine. Moreover, activation of mammalian target of rapamycin complex 1 (mTORC1) signaling by leucine is potentiated by the presence of other essential amino acids (EAA). However, the contribution of the branched-chain amino acids (BCAA) to this effect is yet unknown. Here we compare the stimulatory role of leucine, BCAA, and EAA ingestion on anabolic signaling following exercise. Accordingly, eight trained volunteers completed four sessions of resistance exercise during which they ingested either placebo, leucine, BCAA, or EAA (including the BCAA) in random order. Muscle biopsies were taken at rest, immediately after exercise, and following 90 and 180 min of recovery. Following 90 min of recovery the activity of S6 kinase 1 (S6K1) was greater than at rest in all four trials (Placebo
ISSN:0363-6143
1522-1563
1522-1563
DOI:10.1152/ajpcell.00374.2015