Pregnancy rates after artificial insemination with cooled stallion spermatozoa either with or without Single Layer Centrifugation

A successful outcome after artificial insemination with cooled semen is dependent on many factors, the sperm quality of the ejaculate being one. Previous studies have shown that spermatozoa with good motility, normal morphology, and good chromatin integrity can be selected by means of colloid centri...

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Bibliographic Details
Published in:Theriogenology 2014-11, Vol.82 (8), p.1102-1105
Main Authors: Morrell, J.M., Richter, J., Martinsson, G., Stuhtmann, G., Hoogewijs, M., Roels, K., Dalin, A.-M.
Format: Article
Language:English
Subjects:
Androcoll-E
Animals
Annan veterinärmedicin
Breeding - methods
Cell Separation
Centrifugation - methods
Centrifugation - veterinary
Cold Temperature
Colloids
Cooled stallion semen
Female
Fertility
Horses - physiology
Insemination, Artificial - methods
Insemination, Artificial - statistics & numerical data
Insemination, Artificial - veterinary
Male
Normal ejaculates
Other Veterinary Science
Pregnancy
Pregnancy Rate
Spermatozoa - cytology
Spermatozoa - physiology
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Summary:A successful outcome after artificial insemination with cooled semen is dependent on many factors, the sperm quality of the ejaculate being one. Previous studies have shown that spermatozoa with good motility, normal morphology, and good chromatin integrity can be selected by means of colloid centrifugation, particularly single layer centrifugation (SLC) using species-specific colloids. The purpose of the present study was to conduct an insemination trial with spermatozoa from “normal” ejaculates, i.e., from stallions with no known fertility problem, to determine whether the improvements in sperm quality seen in SLC-selected sperm samples compared with uncentrifuged controls in laboratory tests are reflected in an increased pregnancy rate after artificial insemination. In a multicentre study, SLC-selected sperm samples and uncentrifuged controls from eight stallions were inseminated into approximately 10 mares per treatment per stallion. Ultrasound examination was carried out approximately 16 days after insemination to detect an embryonic vesicle. The pregnancy rates per cycle were 45% for controls and 69% for SLC-selected sperm samples, which is statistically significant (P 
ISSN:0093-691X
1879-3231
1879-3231
DOI:10.1016/j.theriogenology.2014.07.028