Spatial-CUT&Tag: Spatially resolved chromatin modification profiling at the cellular level

Spatial omics emerged as a new frontier of biological and biomedical research. Here, we present spatial-CUT&Tag for spatially resolved genome-wide profiling of histone modifications by combining in situ CUT&Tag chemistry, microfluidic deterministic barcoding, and next-generation sequencing....

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 2022-02, Vol.375 (6581), p.681-686
Main Authors: Deng, Yanxiang, Bartosovic, Marek, Kukanja, Petra, Zhang, Di, Liu, Yang, Su, Graham, Enninful, Archibald, Bai, Zhiliang, Castelo-Branco, Gonçalo, Fan, Rong
Format: Article
Language:English
Subjects:
Animals
Brain - cytology
Brain - embryology
Brain - growth & development
Brain - metabolism
Cell Nucleus - metabolism
Chromatin
Chromatin - metabolism
Embryogenesis
Epigenesis, Genetic
Epigenetics
Epigenome
Gene expression
Gene mapping
Genomes
High-Throughput Nucleotide Sequencing
Histone Code
Histones
Histones - metabolism
Medicin och hälsovetenskap
Mice
Microfluidics
Neurons - cytology
Organogenesis
Pixels
Single-Cell Analysis
Spatial discrimination
Spatial resolution
Transcriptomics
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Summary:Spatial omics emerged as a new frontier of biological and biomedical research. Here, we present spatial-CUT&Tag for spatially resolved genome-wide profiling of histone modifications by combining in situ CUT&Tag chemistry, microfluidic deterministic barcoding, and next-generation sequencing. Spatially resolved chromatin states in mouse embryos revealed tissue-type-specific epigenetic regulations in concordance with ENCODE references and provide spatial information at tissue scale. Spatial-CUT&Tag revealed epigenetic control of the cortical layer development and spatial patterning of cell types determined by histone modification in mouse brain. Single-cell epigenomes can be derived in situ by identifying 20-micrometer pixels containing only one nucleus using immunofluorescence imaging. Spatial chromatin modification profiling in tissue may offer new opportunities to study epigenetic regulation, cell function, and fate decision in normal physiology and pathogenesis.
ISSN:0036-8075
1095-9203
1095-9203
DOI:10.1126/science.abg7216