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Rapid Bedside Inactivation of Ebola Virus for Safe Nucleic Acid Tests
Rapid bedside inactivation of Ebola virus would be a solution for the safety of medical and technical staff, risk containment, sample transport, and high-throughput or rapid diagnostic testing during an outbreak. We show that the commercially available Magna Pure lysis/binding buffer used for nuclei...
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Published in: | Journal of clinical microbiology 2016-10, Vol.54 (10), p.2521-2529 |
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container_title | Journal of clinical microbiology |
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creator | Rosenstierne, Maiken Worsøe Karlberg, Helen Bragstad, Karoline Lindegren, Gunnel Stoltz, Malin Lundahl Salata, Cristiano Kran, Anne-Marte Bakken Dudman, Susanne Gjeruldsen Mirazimi, Ali Fomsgaard, Anders |
description | Rapid bedside inactivation of Ebola virus would be a solution for the safety of medical and technical staff, risk containment, sample transport, and high-throughput or rapid diagnostic testing during an outbreak. We show that the commercially available Magna Pure lysis/binding buffer used for nucleic acid extraction inactivates Ebola virus. A rapid bedside inactivation method for nucleic acid tests is obtained by simply adding Magna Pure lysis/binding buffer directly into vacuum blood collection EDTA tubes using a thin needle and syringe prior to sampling. The ready-to-use inactivation vacuum tubes are stable for more than 4 months, and Ebola virus RNA is preserved in the Magna Pure lysis/binding buffer for at least 5 weeks independent of the storage temperature. We also show that Ebola virus RNA can be manually extracted from Magna Pure lysis/binding buffer-inactivated samples using the QIAamp viral RNA minikit. We present an easy and convenient method for bedside inactivation using available blood collection vacuum tubes and reagents. We propose to use this simple method for fast, safe, and easy bedside inactivation of Ebola virus for safe transport and routine nucleic acid detection. |
doi_str_mv | 10.1128/JCM.00346-16 |
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J.</contributor><creatorcontrib>Rosenstierne, Maiken Worsøe ; Karlberg, Helen ; Bragstad, Karoline ; Lindegren, Gunnel ; Stoltz, Malin Lundahl ; Salata, Cristiano ; Kran, Anne-Marte Bakken ; Dudman, Susanne Gjeruldsen ; Mirazimi, Ali ; Fomsgaard, Anders ; Loeffelholz, M. J.</creatorcontrib><description>Rapid bedside inactivation of Ebola virus would be a solution for the safety of medical and technical staff, risk containment, sample transport, and high-throughput or rapid diagnostic testing during an outbreak. We show that the commercially available Magna Pure lysis/binding buffer used for nucleic acid extraction inactivates Ebola virus. A rapid bedside inactivation method for nucleic acid tests is obtained by simply adding Magna Pure lysis/binding buffer directly into vacuum blood collection EDTA tubes using a thin needle and syringe prior to sampling. The ready-to-use inactivation vacuum tubes are stable for more than 4 months, and Ebola virus RNA is preserved in the Magna Pure lysis/binding buffer for at least 5 weeks independent of the storage temperature. We also show that Ebola virus RNA can be manually extracted from Magna Pure lysis/binding buffer-inactivated samples using the QIAamp viral RNA minikit. We present an easy and convenient method for bedside inactivation using available blood collection vacuum tubes and reagents. We propose to use this simple method for fast, safe, and easy bedside inactivation of Ebola virus for safe transport and routine nucleic acid detection.</description><identifier>ISSN: 0095-1137</identifier><identifier>ISSN: 1098-660X</identifier><identifier>EISSN: 1098-660X</identifier><identifier>DOI: 10.1128/JCM.00346-16</identifier><identifier>PMID: 27466385</identifier><language>eng</language><publisher>United States: American Society for Microbiology</publisher><subject>Disinfection - methods ; Ebolavirus ; Hemorrhagic Fever, Ebola - diagnosis ; Hemorrhagic Fever, Ebola - virology ; Humans ; Medicin och hälsovetenskap ; Point-of-Care Systems ; RNA, Viral - isolation & purification ; Specimen Handling - methods ; Temperature ; Time Factors ; Virology ; Virus Inactivation</subject><ispartof>Journal of clinical microbiology, 2016-10, Vol.54 (10), p.2521-2529</ispartof><rights>Copyright © 2016, American Society for Microbiology. All Rights Reserved.</rights><rights>Copyright © 2016, American Society for Microbiology. All Rights Reserved. 2016 American Society for Microbiology</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c505t-e8121582bceae1e0dabe39f89bf30143b154c4960c1097faf535d5e2dd18a09a3</citedby><cites>FETCH-LOGICAL-c505t-e8121582bceae1e0dabe39f89bf30143b154c4960c1097faf535d5e2dd18a09a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5035422/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5035422/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,3188,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27466385$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttp://kipublications.ki.se/Default.aspx?queryparsed=id:134461557$$DView record from Swedish Publication Index$$Hfree_for_read</backlink></links><search><contributor>Loeffelholz, M. J.</contributor><creatorcontrib>Rosenstierne, Maiken Worsøe</creatorcontrib><creatorcontrib>Karlberg, Helen</creatorcontrib><creatorcontrib>Bragstad, Karoline</creatorcontrib><creatorcontrib>Lindegren, Gunnel</creatorcontrib><creatorcontrib>Stoltz, Malin Lundahl</creatorcontrib><creatorcontrib>Salata, Cristiano</creatorcontrib><creatorcontrib>Kran, Anne-Marte Bakken</creatorcontrib><creatorcontrib>Dudman, Susanne Gjeruldsen</creatorcontrib><creatorcontrib>Mirazimi, Ali</creatorcontrib><creatorcontrib>Fomsgaard, Anders</creatorcontrib><title>Rapid Bedside Inactivation of Ebola Virus for Safe Nucleic Acid Tests</title><title>Journal of clinical microbiology</title><addtitle>J Clin Microbiol</addtitle><description>Rapid bedside inactivation of Ebola virus would be a solution for the safety of medical and technical staff, risk containment, sample transport, and high-throughput or rapid diagnostic testing during an outbreak. We show that the commercially available Magna Pure lysis/binding buffer used for nucleic acid extraction inactivates Ebola virus. A rapid bedside inactivation method for nucleic acid tests is obtained by simply adding Magna Pure lysis/binding buffer directly into vacuum blood collection EDTA tubes using a thin needle and syringe prior to sampling. The ready-to-use inactivation vacuum tubes are stable for more than 4 months, and Ebola virus RNA is preserved in the Magna Pure lysis/binding buffer for at least 5 weeks independent of the storage temperature. We also show that Ebola virus RNA can be manually extracted from Magna Pure lysis/binding buffer-inactivated samples using the QIAamp viral RNA minikit. We present an easy and convenient method for bedside inactivation using available blood collection vacuum tubes and reagents. We propose to use this simple method for fast, safe, and easy bedside inactivation of Ebola virus for safe transport and routine nucleic acid detection.</description><subject>Disinfection - methods</subject><subject>Ebolavirus</subject><subject>Hemorrhagic Fever, Ebola - diagnosis</subject><subject>Hemorrhagic Fever, Ebola - virology</subject><subject>Humans</subject><subject>Medicin och hälsovetenskap</subject><subject>Point-of-Care Systems</subject><subject>RNA, Viral - isolation & purification</subject><subject>Specimen Handling - methods</subject><subject>Temperature</subject><subject>Time Factors</subject><subject>Virology</subject><subject>Virus Inactivation</subject><issn>0095-1137</issn><issn>1098-660X</issn><issn>1098-660X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><recordid>eNqNkjtvFDEUhS0EIkugo0YuKZjg69d6GqSwWiAogAQB0Vke-w4YZseLPRPEv8fJbgIpkKhs2d8596FDyENgRwDcPH29enPEmJC6AX2LLIC1ptGafb5NFoy1qgEQywNyr5RvjIGUSt0lB3wptRZGLcj6vdvGQJ9jKDEgPRmdn-K5m2IaaerpukuDo59ingvtU6YfXI_07ewHjJ4e-6o8wzKV--RO74aCD_bnIfn4Yn22etWcvnt5sjo-bbxiamrQAAdleOfRISALrkPR9qbtelF7Ex0o6WWrma9TLHvXK6GCQh4CGMdaJw5Js_MtP3E7d3ab48blXza5aPdP3-sNrWJCcV359p_8NqfwR3QlBCGlBqWWVftsp63ABoPHccpuuGlx42eMX-2XdH5ZW3JeDR7vDXL6Mdc12U0sHofBjZjmYsFIrkAoY_4D5YIJDlxW9MkO9TmVkrG_7giYvQiErYGwl4GwcLGBR39PcQ1fJUD8BiNGsXY</recordid><startdate>20161001</startdate><enddate>20161001</enddate><creator>Rosenstierne, Maiken Worsøe</creator><creator>Karlberg, Helen</creator><creator>Bragstad, Karoline</creator><creator>Lindegren, Gunnel</creator><creator>Stoltz, Malin Lundahl</creator><creator>Salata, Cristiano</creator><creator>Kran, Anne-Marte Bakken</creator><creator>Dudman, Susanne Gjeruldsen</creator><creator>Mirazimi, Ali</creator><creator>Fomsgaard, Anders</creator><general>American Society for Microbiology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7TM</scope><scope>7U9</scope><scope>H94</scope><scope>5PM</scope><scope>ADTPV</scope><scope>AOWAS</scope><scope>D8T</scope><scope>ZZAVC</scope></search><sort><creationdate>20161001</creationdate><title>Rapid Bedside Inactivation of Ebola Virus for Safe Nucleic Acid Tests</title><author>Rosenstierne, Maiken Worsøe ; Karlberg, Helen ; Bragstad, Karoline ; Lindegren, Gunnel ; Stoltz, Malin Lundahl ; Salata, Cristiano ; Kran, Anne-Marte Bakken ; Dudman, Susanne Gjeruldsen ; Mirazimi, Ali ; Fomsgaard, Anders</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c505t-e8121582bceae1e0dabe39f89bf30143b154c4960c1097faf535d5e2dd18a09a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Disinfection - methods</topic><topic>Ebolavirus</topic><topic>Hemorrhagic Fever, Ebola - diagnosis</topic><topic>Hemorrhagic Fever, Ebola - virology</topic><topic>Humans</topic><topic>Medicin och hälsovetenskap</topic><topic>Point-of-Care Systems</topic><topic>RNA, Viral - isolation & purification</topic><topic>Specimen Handling - methods</topic><topic>Temperature</topic><topic>Time Factors</topic><topic>Virology</topic><topic>Virus Inactivation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rosenstierne, Maiken Worsøe</creatorcontrib><creatorcontrib>Karlberg, Helen</creatorcontrib><creatorcontrib>Bragstad, Karoline</creatorcontrib><creatorcontrib>Lindegren, Gunnel</creatorcontrib><creatorcontrib>Stoltz, Malin Lundahl</creatorcontrib><creatorcontrib>Salata, Cristiano</creatorcontrib><creatorcontrib>Kran, Anne-Marte Bakken</creatorcontrib><creatorcontrib>Dudman, Susanne Gjeruldsen</creatorcontrib><creatorcontrib>Mirazimi, Ali</creatorcontrib><creatorcontrib>Fomsgaard, Anders</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><collection>SwePub</collection><collection>SwePub Articles</collection><collection>SWEPUB Freely available online</collection><collection>SwePub Articles full text</collection><jtitle>Journal of clinical microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rosenstierne, Maiken Worsøe</au><au>Karlberg, Helen</au><au>Bragstad, Karoline</au><au>Lindegren, Gunnel</au><au>Stoltz, Malin Lundahl</au><au>Salata, Cristiano</au><au>Kran, Anne-Marte Bakken</au><au>Dudman, Susanne Gjeruldsen</au><au>Mirazimi, Ali</au><au>Fomsgaard, Anders</au><au>Loeffelholz, M. J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rapid Bedside Inactivation of Ebola Virus for Safe Nucleic Acid Tests</atitle><jtitle>Journal of clinical microbiology</jtitle><addtitle>J Clin Microbiol</addtitle><date>2016-10-01</date><risdate>2016</risdate><volume>54</volume><issue>10</issue><spage>2521</spage><epage>2529</epage><pages>2521-2529</pages><issn>0095-1137</issn><issn>1098-660X</issn><eissn>1098-660X</eissn><abstract>Rapid bedside inactivation of Ebola virus would be a solution for the safety of medical and technical staff, risk containment, sample transport, and high-throughput or rapid diagnostic testing during an outbreak. We show that the commercially available Magna Pure lysis/binding buffer used for nucleic acid extraction inactivates Ebola virus. A rapid bedside inactivation method for nucleic acid tests is obtained by simply adding Magna Pure lysis/binding buffer directly into vacuum blood collection EDTA tubes using a thin needle and syringe prior to sampling. The ready-to-use inactivation vacuum tubes are stable for more than 4 months, and Ebola virus RNA is preserved in the Magna Pure lysis/binding buffer for at least 5 weeks independent of the storage temperature. We also show that Ebola virus RNA can be manually extracted from Magna Pure lysis/binding buffer-inactivated samples using the QIAamp viral RNA minikit. We present an easy and convenient method for bedside inactivation using available blood collection vacuum tubes and reagents. We propose to use this simple method for fast, safe, and easy bedside inactivation of Ebola virus for safe transport and routine nucleic acid detection.</abstract><cop>United States</cop><pub>American Society for Microbiology</pub><pmid>27466385</pmid><doi>10.1128/JCM.00346-16</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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source | ASM Journals (American Society for Microbiology); PubMed Central |
subjects | Disinfection - methods Ebolavirus Hemorrhagic Fever, Ebola - diagnosis Hemorrhagic Fever, Ebola - virology Humans Medicin och hälsovetenskap Point-of-Care Systems RNA, Viral - isolation & purification Specimen Handling - methods Temperature Time Factors Virology Virus Inactivation |
title | Rapid Bedside Inactivation of Ebola Virus for Safe Nucleic Acid Tests |
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