Apoptosis of human ovarian tissue is not increased by either vitrification or rapid cooling

Abstract This study evaluated the incidence of morphological changes, as assessed by light microscopy, and apoptosis in vitrified and rapidly cooled human ovarian tissue. Apoptosis was assessed 30 min and 24 h after warming using transmission electron microscopy, terminal deoxynucleotidyl transferas...

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Published in:Reproductive biomedicine online 2012-11, Vol.25 (5), p.492-499
Main Authors: Salehnia, Mojdeh, Sheikhi, Mona, Pourbeiranvand, Shahram, Lundqvist, Monalill
Format: Article
Language:English
Subjects:
Apoptosis
Cryopreservation - methods
DNA Fragmentation
DNA laddering
Female
Humans
In Situ Nick-End Labeling
in-vitro culture
Medicin och hälsovetenskap
Microscopy, Electron, Transmission
Obstetrics and Gynecology
Ovarian Follicle - pathology
Ovarian Follicle - ultrastructure
ovarian tissue
Ovary - pathology
Ovary - ultrastructure
TUNEL assay
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Summary:Abstract This study evaluated the incidence of morphological changes, as assessed by light microscopy, and apoptosis in vitrified and rapidly cooled human ovarian tissue. Apoptosis was assessed 30 min and 24 h after warming using transmission electron microscopy, terminal deoxynucleotidyl transferase-mediated dUDP nick-end labelling (TUNEL) assay and DNA fragmentation, as determined by gel electrophoresis. The results showed no significant changes in morphology, chromatin condensation, DNA fragmentation or TUNEL-positive cells in follicles attributable to cryopreservation or exposure to the cryoprotectant solutions alone. In conclusion, the cryopreservation protocols did not affect the incidence of apoptosis and either protocol could be an alternative to slow cooling of ovarian tissue. This study evaluated the incidence of morphological changes, as assessed by light microscopy, and apoptosis in human ovarian tissue cryopreserved using two different methods, i.e. vitrification and rapid cooling. Apoptosis was assessed in tissue 30 min and 24 h after warming using transmission electron microscopy and terminal deoxynucleotidyl transferase-mediated dUDP nick-end labelling (TUNEL) assay and DNA fragmentation as determined by gel electrophoresis. The results showed no significant changes in morphology, chromatin condensation, DNA fragmentation or TUNEL-positive cells in follicles attributable to cryopreservation or exposure to the cryopreservation solutions alone. In conclusion, the cryopreservation protocols did not affect the incidence of apoptosis in human ovarian tissue and either protocol could be an alternative to slow cooling for the preservation of ovarian tissue.
ISSN:1472-6483
1472-6491
1472-6491
DOI:10.1016/j.rbmo.2012.07.021