Red Blood Cells Inhibit Proliferation and Stimulate Apoptosis in Human Lung Fibroblasts In Vitro

Cell proliferation and apoptosis are both important mechanisms for the regulation of tissue homeostasis. For instance, proliferation is crucial in wound repair, whereas apoptosis is important for removal of damaged cells and resolution of inflammation. Imbalance between cell proliferation and apopto...

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Bibliographic Details
Published in:Scandinavian journal of immunology 2004-06, Vol.59 (6), p.559-565
Main Authors: Fredriksson, K., Stridh, H., Lundahl, J., Rennard, S. I., Skold, C. M.
Format: Article
Language:English
Subjects:
Apoptosis - physiology
Cell Communication - physiology
Cell Division - physiology
Cells, Cultured
Culture Media, Conditioned
Erythrocytes - physiology
Fibroblasts - pathology
Flow Cytometry
Homeostasis
Humans
In Situ Nick-End Labeling
Lung - cytology
Lung - immunology
Medicin och hälsovetenskap
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Summary:Cell proliferation and apoptosis are both important mechanisms for the regulation of tissue homeostasis. For instance, proliferation is crucial in wound repair, whereas apoptosis is important for removal of damaged cells and resolution of inflammation. Imbalance between cell proliferation and apoptosis can therefore lead to pathological conditions and disease. In inflammatory and fibrotic lung disorders, red blood cells (RBCs) can interact with fibroblasts and connective tissue. In the present study, we therefore hypothesized that the presence of RBCs can affect fibroblast proliferation and apoptosis. Human foetal lung fibroblasts (HFL‐1) were cultured in the presence or absence of purified whole RBCs and RBC‐conditioned media. RBC significantly decreased fibroblast proliferation as determined both by DNA content analysis (Hoechst 33258 staining, P 
ISSN:0300-9475
1365-3083
DOI:10.1111/j.1365-3083.2004.01433.x