Identification of fungal oxaloacetate hydrolyase within the isocitrate lyase/PEP mutase enzyme superfamily using a sequence marker-based method

Aspergillus niger produces oxalic acid through the hydrolysis of oxaloacetate, catalyzed by the cytoplasmic enzyme oxaloacetate acetylhydrolase (OAH). The A. niger genome encodes four additional open reading frames with strong sequence similarity to OAH yet only the oahA gene encodes OAH activity. O...

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Published in:Proteins, structure, function, and bioinformatics structure, function, and bioinformatics, 2008-01, Vol.70 (1), p.157-166
Main Authors: Joosten, Henk-Jan, Han, Ying, Niu, Weiling, Vervoort, Jacques, Dunaway-Mariano, Debra, Schaap, Peter J.
Format: Article
Language:English
Subjects:
Aspergillus niger
Biomarkers - metabolism
Botrytis cinerea
family-based approach
Fungi - classification
Fungi - enzymology
gem-diol
Hydrolases - metabolism
Isocitrate Lyase - genetics
Isocitrate Lyase - metabolism
Kinetics
lyase
multiple protein structural alignment
oxalate production
oxalic-acid biosynthesis
palustris
pathogenicity
petal death protein
plant petal death protein
rolfsii
sclerotinia-sclerotiorum
Substrate Specificity
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Summary:Aspergillus niger produces oxalic acid through the hydrolysis of oxaloacetate, catalyzed by the cytoplasmic enzyme oxaloacetate acetylhydrolase (OAH). The A. niger genome encodes four additional open reading frames with strong sequence similarity to OAH yet only the oahA gene encodes OAH activity. OAH and OAH‐like proteins form subclass of the isocitrate lyase/PEP mutase enzyme superfamily, which is ubiquitous present filamentous fungi. Analysis of function‐specific residues using a superfamily‐based approach revealed an active site serine as a possible sequence marker for OAH activity. We propose that presence of this serine in family members correlates with presence of OAH activity whereas its absence correlates with absence of OAH. This hypothesis was tested by carrying out a serine mutagenesis study with the OAH from the fungal oxalic acid producer Botrytis cinerea and the OAH active plant petal death protein as test systems. Proteins 2008. © 2007 Wiley‐Liss, Inc.
ISSN:0887-3585
1097-0134
DOI:10.1002/prot.21622