Correlation of Rutin Accumulation with 3-O-Glucosyl Transferase and Phenylalanine Ammonia-lyase Activities During the Ripening of Tomato Fruit

• Published in: Plant foods for human nutrition (Dordrecht) 2012-12, Vol.67 (4), p.371-376
• Main Authors: Capanoglu, Esra, Beekwilder, Jules, Matros, Andrea, Boyacioglu, Dilek, Hall, Robert D., Mock, Hans Peter
• Format: Article
• Language: English
• Subjects: anthocyanins; antioxidant; Biological and medical sciences; Chemistry; Chemistry and Materials Science; Chemistry/Food Science; Ecology; esculentum; expression; Food industries; Food Science; Fruit - chemistry; Fruit - enzymology; Fruit - growth & development; Fruit and vegetable industries; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation, Enzymologic; Gene Expression Regulation, Plant; Glucosyltransferases - metabolism; grape berries; induction; Lycopersicon esculentum - chemistry; Lycopersicon esculentum - enzymology; Lycopersicon esculentum - growth & development; metabolites; Nutrition; Organ Specificity; Original Paper; phenolic-compounds; Phenylalanine Ammonia-Lyase - metabolism; Plant Extracts - chemistry; Plant Extracts - metabolism; Plant Physiology; Plant Proteins - isolation & purification; Plant Proteins - metabolism; plants; resistance; Rutin - metabolism
• ISSN: 0921-9668; 1573-9104
• DOI: 10.1007/s11130-012-0321-1

In tomato, the predominant flavonoid is quercetin-3-rutinoside (rutin). In this study, we aim to investigate the phenylalanine ammonia-lyase (PAL) and the quercetin-3- O -glucosyl transferase (3-GT) reactions in the formation of rutin during tomato fruit ripening. Tomatoes of the Moneymaker variety at different development stages (green, breaker, turning, pink, red, and deep red) were divided into flesh and peel fractions. In each sample, both the content of rutin and the enzymatic activities for PAL and 3-GT were recorded. The highest activities of PAL were recorded in the peel of turning fruit (3,000 μkat/mg fresh weight). In fruit flesh, maximal activity was observed in red fruit (917.3 μkat/mg). For both tissues, PAL activity strongly decreased at the final (deep red) fruit stage. The activity of 3-GT in peel peaked in the turning fruit stage (50.7 pkat/mg), while in flesh maximal activity (33.4 pkat/mg) was observed in green fruit, which rapidly declined at the turning stage. Higher levels of rutin were detected in the tomato peel compared to the flesh part with the highest level being found at the green stage. The relation of PAL and 3-GT activities to rutin content is also evaluated.