Characterisation of antioxidant and antiproliferative acidic polysaccharides from Chinese wolfberry fruits

► Wolfberry fruit polysaccharides (WFPs) exhibited dose-dependent antioxidant and antiproliferative effects. ► MCF-7 cells were more responsive to WFP treatment than A549 and LoVo cells. ► WFPs induced apoptosis and arrest at the G0/G1 phase in MCF-7 cells with DNA damage. ► WFPs are involved in an...

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Bibliographic Details
Published in:Food chemistry 2012-08, Vol.133 (3), p.978-989
Main Authors: He, Nianwu, Yang, Xingbin, Jiao, Yadong, Tian, Lingmin, Zhao, Yan
Format: Article
Language:English
Subjects:
anticancer
Antioxidant and antitumor effects
apoptosis
arrest
Biological and medical sciences
breast-cancer cells
cranberry
extraction
Food industries
Fruit and vegetable industries
Fundamental and applied biological sciences. Psychology
growth
in-vitro
lycium-barbarum polysaccharides
Monosaccharide composition
Polysaccharides
prevention
Wolfberry fruit
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Summary:► Wolfberry fruit polysaccharides (WFPs) exhibited dose-dependent antioxidant and antiproliferative effects. ► MCF-7 cells were more responsive to WFP treatment than A549 and LoVo cells. ► WFPs induced apoptosis and arrest at the G0/G1 phase in MCF-7 cells with DNA damage. ► WFPs are involved in an ROS-dependent apoptosis pathway in MCF-7 cells. Wolfberry fruit polysaccharides (WFPs) were isolated by hot-water extraction and ethanol precipitation. With HPLC analysis, WFPs were for the first time identified as acidic polysaccharides with galacturonic acid being the main component monosaccharide (24.9%), followed by galactose (21.3%), arabinose (18.5%), and glucose (15.9%), accounting for up to 80.6% of the molar percentage. WFPs exhibited a high antioxidant activity and a dose-dependent antiproliferative effect, with IC50 values of 134.9, 70.1 and 138.4μg/mL against A549, MCF-7 and LoVo cancer cells after 48h of incubation as estimated by MTT assay, respectively. Flow cytometry analysis showed that WFPs exerted a stimulatory effect on apoptosis of MCF-7 cells, and induced the cell-cycle arrest at the G0/G1 phase, with the observation of intracellular ROS production and DNA damage. The present study demonstrated that these polysaccharides might have the potential to provide significant natural defence against human cancer.
ISSN:0308-8146
1873-7072
DOI:10.1016/j.foodchem.2012.02.018