Influence of yeast immobilization on fermentation and aldehyde reduction during the production of alcohol-free beer

Production of alcohol-free beer by limited fermentation is optimally performed in a packed-bed reactor. This highly controllable system combines short contact times between yeast and wort with the reduction of off-flavors to concentrations below threshold values. In the present study, the influence...

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Bibliographic Details
Published in:Enzyme and microbial technology 2000-05, Vol.26 (8), p.602-607
Main Authors: van Iersel, M.F.M, Brouwer–Post, E, Rombouts, F.M, Abee, T
Format: Article
Language:English
Subjects:
Alcohol-free beer
Aldehydes
Beers
Biological and medical sciences
Biomass
Biosynthesis
Brewer’s yeast
Catalyst activity
DEAE-cellulose
Enzyme kinetics
Fermentation
Fermented food industries
Food industries
Food Microbiology
Food Microbiology Laboratory
Fundamental and applied biological sciences. Psychology
Glucose
Immobilization
Laboratorium Levensmiddelenmicrobiologie
Levensmiddelenmicrobiologie
Reduction
Sugars
VLAG
Yeast
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Summary:Production of alcohol-free beer by limited fermentation is optimally performed in a packed-bed reactor. This highly controllable system combines short contact times between yeast and wort with the reduction of off-flavors to concentrations below threshold values. In the present study, the influence of immobilization of yeast to DEAE-cellulose on sugar fermentation and aldehyde reduction was monitored. Immobilized cells showed higher activities of hexokinase and pyruvate decarboxylase compared to cells grown in batch culture. In addition, a higher glucose flux was observed, with enhanced excretion of main fermentation products, indicating a reduction in the flux of sugar used for biomass production. ADH activity was higher in immobilized cells compared to that in suspended cells. However, during prolonged production a decrease was observed in NAD-specific ADH activity, whereas NADP-specific activity increased in the immobilized cells. The shifts in enzyme activities and glucose flux correlate with a higher in vivo reduction capacity of the immobilized cells.
ISSN:0141-0229
1879-0909
DOI:10.1016/S0141-0229(00)00140-X