Family-level diversity of extracellular proteases of sedimentary bacteria from the South China Sea

Protease-producing bacteria and their extracellular proteases are key players in degrading organic nitrogen to drive marine nitrogen cycling and yet knowledge on both of them is still very limited. This study screened protease-producing bacteria from the South China Sea sediments and analyzed the di...

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Bibliographic Details
Published in:Acta oceanologica Sinica 2019-11, Vol.38 (12), p.73-83
Main Authors: Yang, Jinyu, Feng, Yangyang, Chen, Xiulan, Xie, Binbin, Zhang, Yuzhong, Shi, Mei, Zhang, Xiying
Format: Article
Language:English
Subjects:
Amino acid sequence
Amino acid sequences
Amino acids
Bacteria
Biodegradation
Climatology
DNA
Earth and Environmental Science
Earth Sciences
Ecology
Engineering Fluid Dynamics
Environmental Chemistry
Extracellular
Gene sequencing
Marine & Freshwater Sciences
Microbiological strains
Microorganisms
Nitrogen
Nitrogen cycle
Oceanography
Organic nitrogen
Protease
Proteinase
rRNA 16S
Sediments
Sequence analysis
Sequencing
Serine
Serine proteinase
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Summary:Protease-producing bacteria and their extracellular proteases are key players in degrading organic nitrogen to drive marine nitrogen cycling and yet knowledge on both of them is still very limited. This study screened protease-producing bacteria from the South China Sea sediments and analyzed the diversity of their extracellular proteases at the family level through N-terminal amino acid sequencing. Results of the 16S rRNA gene sequence analysis showed that all screened protease-producing bacteria belonged to the class Gammaproteobacteria and most of them were affiliated with different genera within the orders Alteromonadales and Vibrionales. The N-terminal amino acid sequence analysis for fourteen extracellular proteases from fourteen screened bacterial strains revealed that all these proteases belonged to the M4 family of metalloproteases or the S8 family of serine proteases. This study presents new details on taxa of marine sedimentary protease-producing bacteria and types of their extracellular proteases, which will help to comprehensively understand the process and mechanism of the microbial enzymatic degradation of marine sedimentary organic nitrogen.
ISSN:0253-505X
1869-1099
DOI:10.1007/s13131-019-1391-9