A Novel Schiff Base Zinc Coordination Compound Inhibits Proliferation and Induces Apoptosis of Human Osteosarcoma Cells

• Published in: Journal of Huazhong University of Science and Technology. Medical sciences 2015-10, Vol.35 (5), p.700-706
• Main Author: 闫明 逄利 马坦坦 赵成亮 张楠 郁冰心 夏研
• Format: Article
• Language: English
• Subjects: Antineoplastic Agents - chemical synthesis; Antineoplastic Agents - pharmacology; Apoptosis - drug effects; bcl-2-Associated X Protein - genetics; bcl-2-Associated X Protein - metabolism; Caspase 3 - genetics; Caspase 3 - metabolism; Caspase 8 - genetics; Caspase 8 - metabolism; Caspase 9 - genetics; Caspase 9 - metabolism; caspase-3; Cell Line, Tumor; Cell Proliferation - drug effects; Cell Survival - drug effects; Coordination Complexes - chemical synthesis; Coordination Complexes - pharmacology; Fas Ligand Protein - genetics; Fas Ligand Protein - metabolism; fas Receptor - genetics; fas Receptor - metabolism; G1 Phase Cell Cycle Checkpoints - drug effects; Gene Expression Regulation, Neoplastic - drug effects; Humans; Medicine; Medicine & Public Health; Membrane Potential, Mitochondrial - drug effects; Mitochondria - drug effects; Mitochondria - metabolism; Mitochondria - pathology; Osteoblasts - drug effects; Osteoblasts - metabolism; Osteoblasts - pathology; Proto-Oncogene Proteins c-bcl-2 - genetics; Proto-Oncogene Proteins c-bcl-2 - metabolism; Schiff Bases - chemistry; Signal Transduction; Zinc - chemistry; 希夫碱; 线粒体膜电位; 细胞增殖; 肿瘤细胞凋亡; 诱导凋亡; 锌配合物; 骨肉瘤
• ISSN: 1672-0733; 1993-1352
• DOI: 10.1007/s11596-015-1493-3

Various kinds of schiff base metal complexes have been proven to induce apoptosis of tumor cells. However,it remains largely unknown whether schiff base zinc complexes induce apoptosis in human cancer cells. Here,we synthesized a novel schiff base zinc coordination compound（SBZCC） and investigated its effects on the growth,proliferation and apoptosis of human osteosarcoma MG-63 cells. A novel SBZCC was synthesized by chemical processes and used to treat MG-63 cells. The cell viability was determined by CCK-8 assay. The cell cycle progression,mitochondrial membrane potential and apoptotic cells were analyzed by flow cytometry. The apoptosis-related proteins levels were determined by immunoblotting. Treatment of MG-63 cells with SBZCC resulted in inhibition of cell proliferation and cell cycle arrest at G1 phase. Moreover,SBZCC significantly reduced the mitochondrial membrane potential and induced apoptosis,accompanied with increased Bax/Bcl-2 and Flas L/Fas expression as well as caspase-3/8/9 cleavage. Our results demonstrated that the synthesized novel SBZCC could inhibit the proliferation and induce apoptosis of MG-63 cells via activating both the mitochondrial and cell death receptor apoptosis pathways,suggesting that SBZCC is a promising agent for the development as anticancer drugs.