A competitive microcystin-LR immunosensor based on Au NPs@metal-organic framework (MIL-101)

• Published in: Chinese chemical letters 2019-03, Vol.30 (3), p.664-667
• Main Authors: Zhang, Kunlei, Dai, Kun, Bai, Ruyan, Ma, Yuchan, Deng, Yan, Li, Delei, Zhang, Xi, Hu, Rong, Yang, Yunhui
• Format: Article
• Language: English
• Subjects: Competitive; Electrochemical immunosensor; Metal-organic frameworks; Microcystin-LR; MIL-101
• ISSN: 1001-8417; 1878-5964
• DOI: 10.1016/j.cclet.2018.10.021

A porous metal organic frameworks (MOFs) material (MIL-101) based on trivalent chromium skeleton were synthesized by hydrothermal synthesis method, and loaded with Au nanoparticles (Au NPs) to prepare Au NPs@MIL-101 composite materials which were used as a marker to label anti microcystin-LR (Anti-MC-LR). The composite materials have strong catalytic properties to the oxidation of ascorbic acid. Anti-MC-LR was immobilized on glassy carbon electrode surface using electrodeposition graphene oxide (GO) as a fixed matrix to construct a competitive microcystin-LR immunosensor. [Display omitted] An electrochemical immunosensor was developed for ultrasensitive detection of microcystin-LR in water. MIL-101, a porous metal-organic frameworks (MOFs) material based on trivalent chromium skeleton were synthesized by hydrothermal synthesis method, and loaded with Au nanoparticles (Au NPs) to prepare Au NPs@MIL-101 composite materials which were used as a marker to label anti microcystin-LR (Anti-MC-LR). The composite materials have strong catalytic properties to the oxidation of ascorbic acid. Anti-MC-LR was immobilized on glassy carbon electrode surface using electrodeposition graphene oxide (GO) as an immobilization matrix to construct a competitive microcystin-LR immunosensor. The electrochemical immunosensor display linear relationship in the range of 0.05 ng/mL−75 μg/mL with linear correlation coefficient of 0.9951 and detection limit of 0.02 ng/mL (S/N = 3). This sensor was used to detect microcystin-LR in the water sample. The recovery was 102.43%, which is satisfied. The good testing results indicate the sensor has a great prospect in practical application.