Role of silencing phosphatase of regenerationg liver-3 expression by microRNA interference in the growth of gastric cancer

• Published in: Chinese medical journal 2008-12, Vol.121 (24), p.2534-2538
• Main Authors: Cai, Shi-rong, Wang, Zhao, Chen, Chuang-qi, Wu, Wen-hui, He, Yu-long, Zhan, Wen-hua, Zhang, Chang-hua, Cui, Ji, Wu, Hui
• Format: Article
• Language: English
• Subjects: Blotting, Western; Cell Line, Tumor; Cell Proliferation; Humans; MicroRNAs - genetics; MicroRNAs - physiology; Neoplasm Proteins - genetics; Neoplasm Proteins - metabolism; Neoplasm Proteins - physiology; PRL-3; Protein Tyrosine Phosphatases - genetics; Protein Tyrosine Phosphatases - metabolism; Protein Tyrosine Phosphatases - physiology; Reverse Transcriptase Polymerase Chain Reaction; RNA Interference - physiology; RNA干扰; Stomach Neoplasms - genetics; Stomach Neoplasms - metabolism; 磷酸酶; 胃肿瘤
• ISSN: 0366-6999; 2542-5641

Background Accumulated evidences demonstrate that phosphatase of regeneration liver-3 （PRL-3） is associated with metastasis of multiple tumor types, and has been validated as a potential therapeutic target for metastasis. High expression of PRL-3 was implicated in lymph node metastasis of gastric cancer. In the present study, we investigated the role of silencing PRL-3 expression by microRNA （miRNA） interference in gastric cancer growth. Methods RNA interference, mediated by recombinant lentivirus expressing artificial PRL-3 miRNA, was employed to knockdown PRL-3 expression in human SGC7901 gastric cancer cells. MTT assay and tumor implantation experiment were conducted to determine the role of PRL-3 in the proliferation of SGC7901 cells and tumor growth. Results Transfection of recombinant lentivirus expressing artificial PRL-3 miRNA significantly suppressed the proliferation of SGC7901 cells in vitro. The implanted tumor size of the PRL-3 transfection group was （1.92±0.18） cm^3, significantly smaller compared with controls （P 〈0.001）. Conclusions Knockdown of PRL-3 significantly suppressed the proliferation of SGC7901 cells in vitro and tumor growth in vivo. PRL-3 plays a key role in the growth of gastric cancer. PRL-3 should be considered as a potential therapeutic target.