Upregulation of glucosylceramide synthase protein in papillary thyroid carcinoma

Background Glucosylceramide synthase （GCS） can reduce ceramide levels and help cells escape ceramide-induced apoptosis, thus leading to multidrug resistance （MDR）. However, its expression and clinical significance in thyroid neoplasms still remain unclear. We aimed to elucidate the expression of GCS...

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Bibliographic Details
Published in:Chinese medical journal 2013, Vol.126 (24), p.4660-4664
Main Authors: Zhang, Ke, Song, Ying-Hua, Lin, Xiao-Yan, Wang, Qiang-Xiu, Zhang, Hua-Wei, Xu, Jia-Wen
Format: Article
Language:English
Subjects:
Adult
Blotting, Western
Carcinoma - enzymology
Carcinoma, Papillary
Female
Glucosyltransferases - genetics
Glucosyltransferases - metabolism
Humans
Immunohistochemistry
Male
Prognosis
Retrospective Studies
Thyroid Cancer, Papillary
Thyroid Neoplasms - enzymology
Up-Regulation
Western印迹法
乳头
合酶
甲状腺癌
甲状腺肿瘤
神经酰胺
葡萄糖
蛋白表达
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Summary:Background Glucosylceramide synthase （GCS） can reduce ceramide levels and help cells escape ceramide-induced apoptosis, thus leading to multidrug resistance （MDR）. However, its expression and clinical significance in thyroid neoplasms still remain unclear. We aimed to elucidate the expression of GCS and explore its correlation with the clinicopathological characteristics in papillary thyroid carcinomas （PTCs）. Methods We retrospectively investigated GCS protein expression level in tissue specimens obtained from 108 consecutive PTC patients by immunohistochemistry and Western blotting. Results GCS was weakly positive or negative in normal follicular cells, but it was frequently overexpressed in PTC cells. GCS overexpression was associated with primary tumor size, local infiltration, lymph node metastasis, and local recurrence, but not associated with gender, age, pathological variants, tumor multifocality, tumor stage or distant metastasis. Western blotting also showed that GCS protein levels were much higher in PTCs＇ tissues than in normal thyroid tissues. Conclusion GCS was upregulated in PTCs and might be an independent factor affecting prognosis.
ISSN:0366-6999
2542-5641
DOI:10.3760/cma.j.issn.0366-6999.20131122