A Transgenic Study on Affecting Potato Tuber Yield by Expressing the Rice Sucrose Transporter Genes OsSUT5Z and OsSUT2M

• Published in: Journal of integrative plant biology 2011-07, Vol.53 (7), p.586-595
• Main Authors: Sun, Aijun, Dai, Yan, Zhang, Xinsheng, Li, Chunmin, Meng, Kun, Xu, Honglin, Wei, Xiaoli, Xiao, Guifang, Ouwerkerk, Pieter B.F., Wang, Mei, Zhu, Zhen
• Format: Article
• Language: English
• Subjects: Gene Expression Regulation, Plant - genetics; Gene Expression Regulation, Plant - physiology; Membrane Transport Proteins - genetics; Membrane Transport Proteins - metabolism; Oryza - genetics; Oryza sativa; OsSUT2M; OsSUT5Z; Plant Proteins - genetics; Plant Proteins - metabolism; Plant Tubers - genetics; Plant Tubers - growth & development; Plant Tubers - metabolism; Plants, Genetically Modified - genetics; Plants, Genetically Modified - metabolism; rice sucrose transporter; Solanum tuberosum; Solanum tuberosum - genetics; Solanum tuberosum - growth & development; Solanum tuberosum - metabolism; transgenic potato; tuber yield; 产量影响; 平均体重; 水稻; 蔗糖; 蛋白基因; 转基因; 转运; 马铃薯块茎
• ISSN: 1672-9072; 1744-7909
• DOI: 10.1111/j.1744-7909.2011.01063.x

In many plants, sucrose transporters are essential for both sucrose exports from sources and imports into sinks, indicating a function in assimilate partitioning. To investigate whether sucrose transporters can improve the yield of starch plant, potato plants （Solanum tuberosum L. cv. D~siree） were transformed with cDNAs of the rice sucrose transporter genes OsSUT5Z and OsSUT2M under the control of a tuber- specific, class-I patatin promoter. Compared to the controls, the average fructose content of OsSUTSZ transgenic tubers significantly increased. However, the content of the sugars and starch in the OsSUT2M transgenic potato tubers showed no obvious difference. Correspondingly, the average tuber yield, average number of tubers per plant and average weight of single tuber showed no significant difference in OsSUT2M transgenic tubers with controls. In the OsSUTSZ transgenic lines, the average tuber yield per plant was 1.9-fold higher than the controls, and the average number of tubers per plant increased by more than 10 tubers on average, whereas the average weight of a single tuber did not increase significantly. These results suggested that the average number of tubers per plant showed more contribution than the average weight of a single tuber to the tuber yield per plant.