Advanced glycation end‐products induce oxidative stress through the Sirt1/Nrf2 axis by interacting with the receptor of AGEs under diabetic conditions

• Published in: Journal of cellular biochemistry 2019-02, Vol.120 (2), p.2159-2170
• Main Authors: Chen, Xiao‐Jun, Wu, Wen‐Jun, Zhou, Qi, Jie, Jin‐Ping, Chen, Xiong, Wang, Fang, Gong, Xiao‐Hua
• Format: Article
• Language: English
• Subjects: advanced glycation end‐products; diabetes mellitus; NF‐E2‐related factor 2; oxidative stress; receptor for advanced glycation end products, sirtuin1; thyroid cells
• ISSN: 0730-2312; 1097-4644
• DOI: 10.1002/jcb.27524

Despite the administration of exogenous insulin and other medications used to control many aspects of diabetes mellitus (DM), increased oxidative stress has been increasingly acknowledged in DM development and complications. Therefore, this study aims to investigate the role of advanced glycation end‐products (AGEs) in oxidative stress (OS) of thyroid cells in patients with DM. Patients with DM with or without thyroid dysfunction (TD) were enrolled. Thyroid toxic damage was induced by adding AGE‐modified bovine serum albumin (AGE‐BSA) to normal human thyroid follicular epithelial cells. The cell viability, cell cycle, and cell apoptosis, as well as the content of reactive oxygen species (ROS), catalase (CAT), and malondialdehyde (MDA) in cells were measured. Thyroid hormones, T3, T4, FT3, and FT4 levels were measured by enzyme‐linked immunosorbent assay. Receptor for advanced glycation end products (RAGE), sirtuin1 ( Sirt1), and NF‐E2‐related factor 2 ( Nrf2) expressions were detected, and the mitochondrial membrane potential was measured. We found increased AGEs in the serum of DM patients with TD. By increasing AGE‐BSA concentration, cell viability; the thyroid hormones T3, T4, FT3, and FT4 levels; and mitochondrial membrane potential all significantly decreased. However, the increase in AGE‐BSA concentration led to an increase in cell apoptosis, RAGE, and nuclear factor‐κB expressions but produced the opposite effect on Sirt1, Nrf2, and heme oxygenase‐1 expressions, as well as a decrease in antioxidant response element protein levels. The AGE‐BSA increased ROS and MDA levels and reduced CAT level in normal human thyroid follicular epithelial cells on a dose independence basis. Our results demonstrated that AGEs‐mediated direct increase of RAGE produced OS in thyroid cells of DM by inactivating the Sirt1/Nrf2 axis. Our results demonstrated that advanced glycation end‐products (AGEs)‐mediated direct increase of receptor for advanced glycation end products (RAGE) produced oxidative stress (OS) in thyroid cells of diabetes mellitus (DM) by inactivating the sirtuin1 (Sirt1)/NF‐E2‐related factor 2 (Nrf2) axis.