CO-CULTURE OF DISSOCIATED HIPPOCAMPAL AND SYMPATHETIC CELLS FROM THE NEONATAL RAT

When sympathetic neurones, obtained from superior cervical ganglia of postnatal rats, were grown in microcultures with cells of the postnatal hippocampal formation for 6-44 days, about 70% of the sympathetic neurones formed functional synapses on themselves or a neighbouring sympathetic neurone. In...

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Bibliographic Details
Published in:Experimental physiology 1989-12, Vol.74 (6), p.1043-1050
Main Authors: Johnson, Mark D., Potter, David D.
Format: Article
Language:English
Subjects:
Animals
Animals, Newborn
Biological and medical sciences
Cells, Cultured
Fundamental and applied biological sciences. Psychology
Ganglia, Sympathetic - cytology
Hippocampus - cytology
Isolated neuron and nerve. Neuroglia
Neurons - physiology
Rats
Synapses - physiology
Vertebrates: nervous system and sense organs
Citations: Items that cite this one
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Summary:When sympathetic neurones, obtained from superior cervical ganglia of postnatal rats, were grown in microcultures with cells of the postnatal hippocampal formation for 6-44 days, about 70% of the sympathetic neurones formed functional synapses on themselves or a neighbouring sympathetic neurone. In all forty-four cases in which hexamethonium (0·5-1 mM) was applied it strongly or completely blocked the synaptic interaction. This indicates that the synaptic interaction was cholinergic and raises the possibility that the denervated cells of the hippocampal formation induced the cholinergic function in the co-cultured sympathetic neurones.
ISSN:0958-0670
0144-8757
1469-445X
DOI:10.1113/expphysiol.1989.sp003332