Cell culture‐derived extracellular vesicles: Considerations for reporting cell culturing parameters

Cell culture‐conditioned medium (CCM) is a valuable source of extracellular vesicles (EVs) for basic scientific, therapeutic and diagnostic applications. Cell culturing parameters affect the biochemical composition, release and possibly the function of CCM‐derived EVs (CCM‐EV). The CCM‐EV task force...

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Main Authors: Faezeh Shekari, Faisal J Alibhai, Hossein Baharvand, Verena Börger, Stefania Bruno, Owen Davies, Bernd Giebel, Mario Gimona, Ghasem Hosseini Salekdeh, Lorena Martin‐Jaular, Suresh Mathivanan, Inge Nelissen, Esther Nolte‐’t Hoen, Lorraine O'Driscoll, Francesca Perut, Stefano Pluchino, Gabriella Pocsfalvi, Carlos Salomon, Carolina Soekmadji, Simon Staubach, Ana Claudia Torrecilhas, Ganesh Vilas Shelke, Tobias Tertel, Dandan Zhu, Clotilde Théry, Kenneth Witwer, Rienk Nieuwland
Format: Default Article
Published: 2023
Subjects:
cell culture-conditioned medium
cellular therapy
ectosomes
exosomes
extracellular vesicles
in vitro
reproducibility
rigor
standardization
Online Access:https://hdl.handle.net/2134/24411799.v1
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https://hdl.handle.net/2134/24411799.v1

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