Purification of human prothrombin from Nitschmann fraction III using DEAE membrane radial flow chromatography

A DEAE membrane radial column (4x5 cm) was used to purify human prothrombin from Nitschmann fraction III and performances of the column were investigated. Sample flow-rate of 20-30 ml/min (i.e., 24-36 bed volumes/h) and elution velocity up to 40 ml/min (i.e., 48 bed volumes/h) were obtained without...

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Bibliographic Details
Main Authors: Tao Sun, Ge Chen, Yiping Liu, Fengrong Bu, Meijuan Wen
Format: Default Article
Published: 2000
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Online Access:https://hdl.handle.net/2134/24978
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