Antifoam addition to shake flask cultures of recombinant Pichia pastoris increases yield

Pichia pastoris is a widely-used host for recombinant protein production. Initial screening for both suitable clones and optimum culture conditions is typically carried out in multi-well plates. This is followed by up-scaling either to shake-flasks or continuously stirred tank bioreactors. A particu...

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Bibliographic Details
Main Authors: Sarah J. Routledge, Christopher Hewitt, Nagamani Bora, Roslyn M. Bill
Format: Default Article
Published: 2011
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Online Access:https://hdl.handle.net/2134/8556
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