Exploitation of GFP fusion proteins and stress avoidance as a generic strategy for the production of high quality recombinant proteins

A C-terminal green fluorescent protein (GFP) fusion to a model target protein, Escherichia coli CheY, was exploited both as a reporter of the accumulation of soluble recombinant protein, and to develop a generic approach to optimize protein yields. The rapid accumulation of CheY∷GFP expressed from a...

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Bibliographic Details

Main Authors:	Yanina Sevastsyanovich, Sara Alfasi, Tim Overton, Richard Hall, Jo Jones, Christopher Hewitt, Jeff Cole
Format:	Default Preprint
Published:	2009
Subjects:	Chemical engineering not elsewhere classified Recombinant protein production Inclusion bodies Gonococcal 1 cytochrome c2 Heat shock General stress response Green fluorescent protein Flow cytometry Chemical Engineering not elsewhere classified
Online Access:	https://hdl.handle.net/2134/5759
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