Development and characterization of a porcine mitral valve scaffold for tissue engineering

Decellularized scaffolds represent a promising alternative for mitral valve (MV) replacement. This work developed and characterized a protocol for the decellularization of whole MVs. Porcine MVs were decellularized with 0.5% (w/ v) SDS and 0.5% (w/v) SD and sterilized with 0.1% (v/v) PAA. Decellular...

Full description

Saved in:
Bibliographic Details
Main Authors: M. Granados, Lucrezia Morticelli, S. Andriopoulou, P. Kalozoumis, Michael Pflaum, P. Iablonskii, B. Glasmacher, M. Harder, J. Hegermann, C. Wrede, I. Tudorache, S. Cebotari, Andres Hilfiker, Axel Haverich, Sotiris Korossis
Format: Default Article
Published: 2017
Subjects:
Mechanical engineering not elsewhere classified
Mitral valve
Heart valve replacement
Decellularization
Biomechanics
Histology
Immunohistochemistry
Biochemistry
α-Gal
Xenoepitope
Collagen IV
Biocompatibility
Tissue engineering
Scaffold
Transmission electron microscopy
Scanning electron microscopy
Cytotoxicity
Scaffold seeding
Human foreskin fibroblasts
Humanadipose-derived stem cells
Human colony-forming endothelial cells
Mechanical Engineering not elsewhere classified
Online Access:https://hdl.handle.net/2134/37014
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Decellularized scaffolds represent a promising alternative for mitral valve (MV) replacement. This work developed and characterized a protocol for the decellularization of whole MVs. Porcine MVs were decellularized with 0.5% (w/ v) SDS and 0.5% (w/v) SD and sterilized with 0.1% (v/v) PAA. Decellularized samples were seeded with human foreskin fibroblasts and human adipose-derived stem cells to investigate cellular repopulation and infiltration, and with human colonyforming endothelial cells to investigate collagen IV formation. Histology revealed an acellular scaffold with a generally conserved histoarchitecture, but collagen IV loss. Following decellularization, no significant changes were observed in the hydroxyproline content, but there was a significant reduction in the glycosaminoglycan content. SEM/TEM analysis confirmed cellular removal and loss of some extracellular matrix components. Collagen and elastin were generally preserved. The endothelial cells produced newly formed collagen IVon the non-cytotoxic scaffold. The protocol produced acellular scaffolds with generally preserved histoarchitecture, biochemistry, and biomechanics.

Similar Items