Detecting intracellular thiol redox state in leukaemia and heterogeneous immune cell populations: An optimised protocol for digital flow cytometers

Flow cytometric methods for detecting and quantifying reduced intracellular thiol content using fluorescein-5-maleimide (F5M) in viable eukaryotic cells date back to 1983 (Durand and Olive [1]). There has been little development in these methodologies since that time, a period that has witnessed hug...

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Bibliographic Details

Main Authors:	Alex Wadley, Rhys G. Morgan, Kate J. Heesom, Paul S. Hole, Steven J. Coles
Format:	Default Article
Published:	2018
Subjects:	Other health sciences not elsewhere classified Flow cytometry Reactive oxygen species Oxidative stress Fluorescein-5 maleimide Reductive stress Medical and Health Sciences not elsewhere classified
Online Access:	https://hdl.handle.net/2134/36973
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