Loading…
RGMb controls aggregation and migration of Neogenin-positive cells in vitro and in vivo
The proliferation, migration and differentiation of dentate gyrus stem and precursor cells have aroused keen interest. Neogenin and RGMb are expressed in non-overlapping compartments of the developing dentate gyrus. While Neogenin is expressed in migrating and proliferating dentate precursors, RGMb...
Saved in:
Published in: | Molecular and cellular neuroscience 2010-02, Vol.43 (2), p.222-231 |
---|---|
Main Authors: | , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
cited_by | cdi_FETCH-LOGICAL-c384t-8c14f2312ab8826de074cb8e5276fe67793869d5a20f9fb54a5dea024ce3d2b43 |
---|---|
cites | cdi_FETCH-LOGICAL-c384t-8c14f2312ab8826de074cb8e5276fe67793869d5a20f9fb54a5dea024ce3d2b43 |
container_end_page | 231 |
container_issue | 2 |
container_start_page | 222 |
container_title | Molecular and cellular neuroscience |
container_volume | 43 |
creator | Conrad, Sabine Stimpfle, Fabian Montazeri, Sonia Oldekamp, Judit Seid, Karin Alvarez-Bolado, Gonzalo Skutella, Thomas |
description | The proliferation, migration and differentiation of dentate gyrus stem and precursor cells have aroused keen interest. Neogenin and RGMb are expressed in non-overlapping compartments of the developing dentate gyrus. While Neogenin is expressed in migrating and proliferating dentate precursors, RGMb is localized in structures bordering the developing dentate, such as cornus ammonis cells and Cajal–Retzius cells in the marginal zone including the hippocampal fissure. Co-immunoprecipitation and binding assays indicate a strong physical interaction.
In vitro and
in vivo migration of dentate neuroepithelial cells is abolished by RGMb, and cell adhesion is reduced when cells expressing Neogenin comes into contact with cells expressing RGMb. Ectopic expression of RGMb in organotypic slice cultures and after
in utero electroporation in the hippocampus modifies precursor cell migration. Our results imply that Neogenin-RGMb interaction might be involved in neuronal migration in the dentate gyrus. |
doi_str_mv | 10.1016/j.mcn.2009.11.003 |
format | article |
fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_744710808</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S1044743109002425</els_id><sourcerecordid>744710808</sourcerecordid><originalsourceid>FETCH-LOGICAL-c384t-8c14f2312ab8826de074cb8e5276fe67793869d5a20f9fb54a5dea024ce3d2b43</originalsourceid><addsrcrecordid>eNqFkE1r3DAQhkVo6W7S_oBcim892Rl92JboqYRkE0hSKAk9ClkeGy1raSt5F_rvo80u9NacRgPP-454CLmkUFGgzdW6mqyvGICqKK0A-BlZUlB1qThrPxzeQpSt4HRBzlNaA0DNFP9EFlQpIWgjluT3r9VjV9jg5xg2qTDjGHE0swu-ML4vJjfG4xaG4gnDiN75chuSm90eC4ubHHK-2Lucf0u8LfvwmXwczCbhl9O8IC-3N8_Xd-XDz9X99Y-H0nIp5lJaKgbGKTOdlKzpEVphO4k1a5sBm7ZVXDaqrw2DQQ1dLUzdowEmLPKedYJfkG_H3m0Mf3aYZj25dPiW8Rh2SbdCtBQkyPdJzlW-LiGT9EjaGFKKOOhtdJOJfzUFfRCv1zqL1wfxmlKdxefM11P7rpuw_5c4mc7A9yOA2cbeYdTJOvQWexfRzroP7j_1r352kyU</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>733907480</pqid></control><display><type>article</type><title>RGMb controls aggregation and migration of Neogenin-positive cells in vitro and in vivo</title><source>Elsevier</source><creator>Conrad, Sabine ; Stimpfle, Fabian ; Montazeri, Sonia ; Oldekamp, Judit ; Seid, Karin ; Alvarez-Bolado, Gonzalo ; Skutella, Thomas</creator><creatorcontrib>Conrad, Sabine ; Stimpfle, Fabian ; Montazeri, Sonia ; Oldekamp, Judit ; Seid, Karin ; Alvarez-Bolado, Gonzalo ; Skutella, Thomas</creatorcontrib><description>The proliferation, migration and differentiation of dentate gyrus stem and precursor cells have aroused keen interest. Neogenin and RGMb are expressed in non-overlapping compartments of the developing dentate gyrus. While Neogenin is expressed in migrating and proliferating dentate precursors, RGMb is localized in structures bordering the developing dentate, such as cornus ammonis cells and Cajal–Retzius cells in the marginal zone including the hippocampal fissure. Co-immunoprecipitation and binding assays indicate a strong physical interaction.
In vitro and
in vivo migration of dentate neuroepithelial cells is abolished by RGMb, and cell adhesion is reduced when cells expressing Neogenin comes into contact with cells expressing RGMb. Ectopic expression of RGMb in organotypic slice cultures and after
in utero electroporation in the hippocampus modifies precursor cell migration. Our results imply that Neogenin-RGMb interaction might be involved in neuronal migration in the dentate gyrus.</description><identifier>ISSN: 1044-7431</identifier><identifier>EISSN: 1095-9327</identifier><identifier>DOI: 10.1016/j.mcn.2009.11.003</identifier><identifier>PMID: 19944164</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Cell Line, Transformed ; Cell Movement - physiology ; Dentate gyrus development ; Electroporation - methods ; Embryo, Mammalian ; Fluoresceins ; Gene Expression Regulation, Developmental - physiology ; Green Fluorescent Proteins - genetics ; Hippocampus - cytology ; Homeodomain Proteins - metabolism ; Humans ; Immunoprecipitation ; Membrane Proteins - genetics ; Membrane Proteins - metabolism ; Mice ; Mice, Inbred C57BL ; Migration ; Neogenin ; Nerve Tissue Proteins - genetics ; Nerve Tissue Proteins - metabolism ; Neurons - physiology ; Organ Culture Techniques ; RGMb ; Stem Cells - physiology ; Transfection - methods ; Tumor Suppressor Proteins - metabolism</subject><ispartof>Molecular and cellular neuroscience, 2010-02, Vol.43 (2), p.222-231</ispartof><rights>2009 Elsevier Inc.</rights><rights>Copyright 2009 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c384t-8c14f2312ab8826de074cb8e5276fe67793869d5a20f9fb54a5dea024ce3d2b43</citedby><cites>FETCH-LOGICAL-c384t-8c14f2312ab8826de074cb8e5276fe67793869d5a20f9fb54a5dea024ce3d2b43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19944164$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Conrad, Sabine</creatorcontrib><creatorcontrib>Stimpfle, Fabian</creatorcontrib><creatorcontrib>Montazeri, Sonia</creatorcontrib><creatorcontrib>Oldekamp, Judit</creatorcontrib><creatorcontrib>Seid, Karin</creatorcontrib><creatorcontrib>Alvarez-Bolado, Gonzalo</creatorcontrib><creatorcontrib>Skutella, Thomas</creatorcontrib><title>RGMb controls aggregation and migration of Neogenin-positive cells in vitro and in vivo</title><title>Molecular and cellular neuroscience</title><addtitle>Mol Cell Neurosci</addtitle><description>The proliferation, migration and differentiation of dentate gyrus stem and precursor cells have aroused keen interest. Neogenin and RGMb are expressed in non-overlapping compartments of the developing dentate gyrus. While Neogenin is expressed in migrating and proliferating dentate precursors, RGMb is localized in structures bordering the developing dentate, such as cornus ammonis cells and Cajal–Retzius cells in the marginal zone including the hippocampal fissure. Co-immunoprecipitation and binding assays indicate a strong physical interaction.
In vitro and
in vivo migration of dentate neuroepithelial cells is abolished by RGMb, and cell adhesion is reduced when cells expressing Neogenin comes into contact with cells expressing RGMb. Ectopic expression of RGMb in organotypic slice cultures and after
in utero electroporation in the hippocampus modifies precursor cell migration. Our results imply that Neogenin-RGMb interaction might be involved in neuronal migration in the dentate gyrus.</description><subject>Animals</subject><subject>Cell Line, Transformed</subject><subject>Cell Movement - physiology</subject><subject>Dentate gyrus development</subject><subject>Electroporation - methods</subject><subject>Embryo, Mammalian</subject><subject>Fluoresceins</subject><subject>Gene Expression Regulation, Developmental - physiology</subject><subject>Green Fluorescent Proteins - genetics</subject><subject>Hippocampus - cytology</subject><subject>Homeodomain Proteins - metabolism</subject><subject>Humans</subject><subject>Immunoprecipitation</subject><subject>Membrane Proteins - genetics</subject><subject>Membrane Proteins - metabolism</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Migration</subject><subject>Neogenin</subject><subject>Nerve Tissue Proteins - genetics</subject><subject>Nerve Tissue Proteins - metabolism</subject><subject>Neurons - physiology</subject><subject>Organ Culture Techniques</subject><subject>RGMb</subject><subject>Stem Cells - physiology</subject><subject>Transfection - methods</subject><subject>Tumor Suppressor Proteins - metabolism</subject><issn>1044-7431</issn><issn>1095-9327</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><recordid>eNqFkE1r3DAQhkVo6W7S_oBcim892Rl92JboqYRkE0hSKAk9ClkeGy1raSt5F_rvo80u9NacRgPP-454CLmkUFGgzdW6mqyvGICqKK0A-BlZUlB1qThrPxzeQpSt4HRBzlNaA0DNFP9EFlQpIWgjluT3r9VjV9jg5xg2qTDjGHE0swu-ML4vJjfG4xaG4gnDiN75chuSm90eC4ubHHK-2Lucf0u8LfvwmXwczCbhl9O8IC-3N8_Xd-XDz9X99Y-H0nIp5lJaKgbGKTOdlKzpEVphO4k1a5sBm7ZVXDaqrw2DQQ1dLUzdowEmLPKedYJfkG_H3m0Mf3aYZj25dPiW8Rh2SbdCtBQkyPdJzlW-LiGT9EjaGFKKOOhtdJOJfzUFfRCv1zqL1wfxmlKdxefM11P7rpuw_5c4mc7A9yOA2cbeYdTJOvQWexfRzroP7j_1r352kyU</recordid><startdate>20100201</startdate><enddate>20100201</enddate><creator>Conrad, Sabine</creator><creator>Stimpfle, Fabian</creator><creator>Montazeri, Sonia</creator><creator>Oldekamp, Judit</creator><creator>Seid, Karin</creator><creator>Alvarez-Bolado, Gonzalo</creator><creator>Skutella, Thomas</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7TK</scope></search><sort><creationdate>20100201</creationdate><title>RGMb controls aggregation and migration of Neogenin-positive cells in vitro and in vivo</title><author>Conrad, Sabine ; Stimpfle, Fabian ; Montazeri, Sonia ; Oldekamp, Judit ; Seid, Karin ; Alvarez-Bolado, Gonzalo ; Skutella, Thomas</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c384t-8c14f2312ab8826de074cb8e5276fe67793869d5a20f9fb54a5dea024ce3d2b43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Animals</topic><topic>Cell Line, Transformed</topic><topic>Cell Movement - physiology</topic><topic>Dentate gyrus development</topic><topic>Electroporation - methods</topic><topic>Embryo, Mammalian</topic><topic>Fluoresceins</topic><topic>Gene Expression Regulation, Developmental - physiology</topic><topic>Green Fluorescent Proteins - genetics</topic><topic>Hippocampus - cytology</topic><topic>Homeodomain Proteins - metabolism</topic><topic>Humans</topic><topic>Immunoprecipitation</topic><topic>Membrane Proteins - genetics</topic><topic>Membrane Proteins - metabolism</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Migration</topic><topic>Neogenin</topic><topic>Nerve Tissue Proteins - genetics</topic><topic>Nerve Tissue Proteins - metabolism</topic><topic>Neurons - physiology</topic><topic>Organ Culture Techniques</topic><topic>RGMb</topic><topic>Stem Cells - physiology</topic><topic>Transfection - methods</topic><topic>Tumor Suppressor Proteins - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Conrad, Sabine</creatorcontrib><creatorcontrib>Stimpfle, Fabian</creatorcontrib><creatorcontrib>Montazeri, Sonia</creatorcontrib><creatorcontrib>Oldekamp, Judit</creatorcontrib><creatorcontrib>Seid, Karin</creatorcontrib><creatorcontrib>Alvarez-Bolado, Gonzalo</creatorcontrib><creatorcontrib>Skutella, Thomas</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Neurosciences Abstracts</collection><jtitle>Molecular and cellular neuroscience</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Conrad, Sabine</au><au>Stimpfle, Fabian</au><au>Montazeri, Sonia</au><au>Oldekamp, Judit</au><au>Seid, Karin</au><au>Alvarez-Bolado, Gonzalo</au><au>Skutella, Thomas</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>RGMb controls aggregation and migration of Neogenin-positive cells in vitro and in vivo</atitle><jtitle>Molecular and cellular neuroscience</jtitle><addtitle>Mol Cell Neurosci</addtitle><date>2010-02-01</date><risdate>2010</risdate><volume>43</volume><issue>2</issue><spage>222</spage><epage>231</epage><pages>222-231</pages><issn>1044-7431</issn><eissn>1095-9327</eissn><abstract>The proliferation, migration and differentiation of dentate gyrus stem and precursor cells have aroused keen interest. Neogenin and RGMb are expressed in non-overlapping compartments of the developing dentate gyrus. While Neogenin is expressed in migrating and proliferating dentate precursors, RGMb is localized in structures bordering the developing dentate, such as cornus ammonis cells and Cajal–Retzius cells in the marginal zone including the hippocampal fissure. Co-immunoprecipitation and binding assays indicate a strong physical interaction.
In vitro and
in vivo migration of dentate neuroepithelial cells is abolished by RGMb, and cell adhesion is reduced when cells expressing Neogenin comes into contact with cells expressing RGMb. Ectopic expression of RGMb in organotypic slice cultures and after
in utero electroporation in the hippocampus modifies precursor cell migration. Our results imply that Neogenin-RGMb interaction might be involved in neuronal migration in the dentate gyrus.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>19944164</pmid><doi>10.1016/j.mcn.2009.11.003</doi><tpages>10</tpages></addata></record> |
fulltext | fulltext |
identifier | ISSN: 1044-7431 |
ispartof | Molecular and cellular neuroscience, 2010-02, Vol.43 (2), p.222-231 |
issn | 1044-7431 1095-9327 |
language | eng |
recordid | cdi_proquest_miscellaneous_744710808 |
source | Elsevier |
subjects | Animals Cell Line, Transformed Cell Movement - physiology Dentate gyrus development Electroporation - methods Embryo, Mammalian Fluoresceins Gene Expression Regulation, Developmental - physiology Green Fluorescent Proteins - genetics Hippocampus - cytology Homeodomain Proteins - metabolism Humans Immunoprecipitation Membrane Proteins - genetics Membrane Proteins - metabolism Mice Mice, Inbred C57BL Migration Neogenin Nerve Tissue Proteins - genetics Nerve Tissue Proteins - metabolism Neurons - physiology Organ Culture Techniques RGMb Stem Cells - physiology Transfection - methods Tumor Suppressor Proteins - metabolism |
title | RGMb controls aggregation and migration of Neogenin-positive cells in vitro and in vivo |
url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-29T19%3A11%3A10IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=RGMb%20controls%20aggregation%20and%20migration%20of%20Neogenin-positive%20cells%20in%20vitro%20and%20in%20vivo&rft.jtitle=Molecular%20and%20cellular%20neuroscience&rft.au=Conrad,%20Sabine&rft.date=2010-02-01&rft.volume=43&rft.issue=2&rft.spage=222&rft.epage=231&rft.pages=222-231&rft.issn=1044-7431&rft.eissn=1095-9327&rft_id=info:doi/10.1016/j.mcn.2009.11.003&rft_dat=%3Cproquest_cross%3E744710808%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c384t-8c14f2312ab8826de074cb8e5276fe67793869d5a20f9fb54a5dea024ce3d2b43%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=733907480&rft_id=info:pmid/19944164&rfr_iscdi=true |